| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ferris, Josephine E (13)
|
PVMACH |
26.73 |
28.17 |
1.44 |
| 2 |
Blake, Emilia B (13)
|
PVMACH |
27.54 |
28.32 |
0.78 |
| 3 |
Lee-Truesdell, Isabella S (14)
|
PVMACH |
27.91 |
28.46 |
0.55 |
| 4 |
English, Ella W (13)
|
PVMACH |
27.65 |
28.49 |
0.84 |
| 5 |
Paulson, Valeria R (13)
|
PVMACH |
21.13 |
28.64 |
7.51 |
| 6 |
Nagle, Audrey R (13)
|
PVMACH |
30.25 |
30.28 |
0.03 |
| 7 |
Canaday, Audrey A (13)
|
PVMACH |
29.34 |
30.29 |
0.95 |
| 8 |
Blachere, Corinne S (13)
|
PVMACH |
30.06 |
30.53 |
0.47 |
| 9 |
Ebaugh, Madison E (13)
|
PVMACH |
28.94 |
30.59 |
1.65 |
| 10 |
Faustini, Nina M (13)
|
PVMACH |
29.71 |
30.91 |
1.20 |
| 11 |
Ladd, Kennedy M (14)
|
PVMACH |
30.70 |
31.48 |
0.78 |
| 12 |
Kasemsuwannakaeth, Natcha (13)
|
PVMACH |
30.73 |
31.52 |
0.79 |
| 13 |
Tohmeh, Tala E (13)
|
PVMACH |
32.42 |
32.44 |
0.02 |
| 14 |
Arllen, Sydney M (13)
|
PVMACH |
|
32.84 |
- |
|
Li, Samantha W (13)
|
PVMACH |
31.78 |
NS |
- |