| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
kil, Isabella C (13)
|
MAACWS |
2:32.46 |
2:33.41 |
0.95 |
| 2 |
Park, Jordynn D (14)
|
MAEAC |
2:33.10 |
2:33.45 |
0.35 |
| 3 |
Lyesnykova, Alina V (14)
|
MACVAC |
2:34.74 |
2:36.39 |
1.65 |
| 4 |
Cottingham, Brianna A (14)
|
MASCAY |
2:42.48 |
2:37.69 |
-4.79 |
| 5 |
Pagett, Mackenzie S (13)
|
MASCAY |
2:38.66 |
2:37.95 |
-0.71 |
| 6 |
Turner, Sara C (13)
|
MAEPAC |
2:37.99 |
2:41.17 |
3.18 |
| 7 |
Root, Ella A (14)
|
MAMARA |
2:39.78 |
2:41.77 |
1.99 |
| 8 |
Deyrup, Caroline V (14)
|
MABRY |
2:37.05 |
2:52.22 |
15.17 |
| 9 |
Mulveny, Leila M (14)
|
MABRY |
2:41.70 |
2:40.03 |
-1.67 |
| 10 |
Holtsberg, Cathryn F (13)
|
MASSC |
2:38.01 |
2:42.13 |
4.12 |
| 11 |
Estep, Mia (13)
|
MAEPAC |
2:47.94 |
2:45.88 |
-2.06 |
| 12 |
Burton, Apolina M (14)
|
MAWSY |
2:43.81 |
2:46.25 |
2.44 |
| 13 |
Brandwene, Sophie J (13)
|
MANLAC |
2:53.43 |
2:49.50 |
-3.93 |
| 14 |
Parks, Kylee E (14)
|
MAHAY |
2:53.19 |
2:50.78 |
-2.41 |
| 15 |
Shifflett, Ella M (13)
|
MASSC |
2:54.36 |
2:53.03 |
-1.33 |
| 16 |
Zimmerman, Hannah M (14)
|
MAEPAC |
2:55.65 |
2:55.11 |
-0.54 |