| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rampersad, Kiera J (12)
|
FGNMST |
34.62 |
34.89 |
0.27 |
| 2 |
Vazquez, Ayelen (11)
|
FGOSC |
|
43.05 |
- |
| 3 |
Regueira, Meghan N (11)
|
FGMACM |
55.50 |
51.49 |
-4.01 |
| 4 |
Merino, Valentina E (11)
|
FGSDST |
|
51.51 |
- |
| 5 |
Cassels, Alexandra M (12)
|
FGMACM |
|
52.01 |
- |
| 6 |
Steiger, Eliana L (12)
|
FGNMST |
59.35 |
56.17 |
-3.18 |
| 7 |
Collazo-Wood, Estefania (12)
|
FGOSC |
|
58.95 |
- |
| 8 |
Zdircu, Alessandra N (11)
|
FGMACM |
|
59.46 |
- |
| 9 |
Jackson, Di'Myah F (12)
|
FGOSC |
|
59.75 |
- |
| 10 |
Zdircu, Isabella N (11)
|
FGMACM |
|
1:00.25 |
- |
| 11 |
Sierra-Soderman, Emma S (11)
|
FGEA |
|
1:04.96 |
- |
|
Cleary, Caitlin S (11)
|
FGCANE |
52.10 |
DQ |
- |