| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Morales, Angelika M (10)
|
FGEA |
46.18 |
45.45 |
-0.73 |
| 2 |
Lisitsyn, Elizabeth (10)
|
FGNMST |
45.27 |
45.67 |
0.40 |
| 3 |
Salazar, Alicia A (9)
|
FGMACM |
48.25 |
48.11 |
-0.14 |
| 4 |
D'Andrea, Victoria G (10)
|
FGMBS |
52.09 |
48.12 |
-3.97 |
| 5 |
Cancio Jorge, Alma N (8)
|
FGMACM |
55.85 |
48.88 |
-6.97 |
| 6 |
Dennis, Janiya M (10)
|
FGFLA |
51.64 |
50.28 |
-1.36 |
| 7 |
Roper, Bree D (8)
|
FGFLA |
1:06.78 |
52.09 |
-14.69 |
| 8 |
Alfaro, Juliet N (10)
|
FGEA |
55.00 |
53.73 |
-1.27 |
| 9 |
David, Antonia (9)
|
FGEA |
54.72 |
54.48 |
-0.24 |
| 10 |
Candelaria, Gabriela E (9)
|
FGMACM |
56.70 |
54.58 |
-2.12 |
| 11 |
Vazquez, Daira (7)
|
FGOSC |
53.99 |
54.89 |
0.90 |
| 12 |
Cuevas, Zoe A (9)
|
FGAJSC |
|
55.98 |
- |
| 13 |
Alfonso, Anabella S (10)
|
FGMS |
|
56.36 |
- |
| 14 |
Abijo, Sofi O (8)
|
FGEA |
58.96 |
56.78 |
-2.18 |
| 15 |
Morales, Anastasia C (6)
|
FGEA |
1:12.32 |
56.99 |
-15.33 |
| 16 |
Kolobova, Eva * (7)
|
FGNMST |
51.86 |
58.58 |
6.72 |
| 17 |
Wolk, Emily S (10)
|
FGMCDA |
1:05.71 |
59.31 |
-6.40 |
| 18 |
Van Mossel, Emma M (10)
|
FGOSC |
1:05.34 |
59.57 |
-5.77 |
| 19 |
Fenot, Rafaelle (9)
|
FGMBS |
|
59.96 |
- |
| 20 |
Spencer-Funchion, Dorothy D (8)
|
FGCANE |
1:02.89 |
1:05.15 |
2.26 |
| 21 |
Barreto, Zoe (7)
|
FGOSC |
1:18.20 |
1:05.22 |
-12.98 |
| 22 |
Albuja, Rebecca M (9)
|
FGEA |
1:04.86 |
1:05.46 |
0.60 |
| 23 |
Orozco, Isabella (10)
|
FGOSC |
|
1:06.87 |
- |
| 24 |
Diaz, Maya (10)
|
FGASC |
59.72 |
1:07.49 |
7.77 |
| 25 |
Alvarez Malpica, Nayaraq A (9)
|
FGMBS |
|
1:08.14 |
- |
| 26 |
Dorta, Simone D (8)
|
FGAJSC |
1:09.77 |
1:11.26 |
1.49 |
| 27 |
Grant, Faith E (9)
|
FGAJSC |
1:00.06 |
1:11.70 |
11.64 |
| 28 |
Fitch, Aiyana L (8)
|
FGFLA |
|
1:15.47 |
- |
| 29 |
Blanco, Alexandra (9)
|
FGASC |
|
1:15.64 |
- |