| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Morales, Angelika M (10)
|
FGEA |
44.18 |
39.95 |
-4.23 |
| 2 |
Nunez-Estrada, Estefania (10)
|
UNAT |
27.47 |
41.97 |
14.50 |
| 3 |
Ospina, Ana S (9)
|
UNAT |
42.46 |
42.09 |
-0.37 |
| 4 |
Bachmann, Kristen S (10)
|
UNAT |
47.17 |
47.85 |
0.68 |
| 5 |
Cancio Jorge, Alma N (8)
|
FGMACM |
55.46 |
49.70 |
-5.76 |
| 6 |
Vazquez, Daira (7)
|
FGOSC |
|
49.75 |
- |
| 7 |
Montesino, Carlotta G (9)
|
FGRANS |
48.82 |
49.96 |
1.14 |
| 8 |
Ramos, Jenica I (9)
|
FGMACM |
58.08 |
51.22 |
-6.86 |
| 9 |
Roper, Bree D (8)
|
FGFLA |
|
51.37 |
- |
| 10 |
Dennis, Janiya M (10)
|
FGFLA |
58.22 |
52.46 |
-5.76 |
| 11 |
O'Hara, Shauna G (8)
|
FGEA |
|
53.12 |
- |
| 12 |
David, Antonia (9)
|
FGEA |
55.02 |
54.23 |
-0.79 |
| 13 |
Kotz-Agramonte, Sofia O (9)
|
FGRANS |
54.01 |
56.02 |
2.01 |
| 14 |
Van Mossel, Emma M (10)
|
FGOSC |
53.14 |
56.15 |
3.01 |
| 15 |
Wolk, Emily S (10)
|
FGMCDA |
54.92 |
56.69 |
1.77 |
| 16 |
Gentilhomme, Lydia Z (10)
|
FGFLA |
1:01.99 |
57.63 |
-4.36 |
| 17 |
Grant, Faith E (9)
|
FGAJSC |
1:01.95 |
58.27 |
-3.68 |
| 18 |
Blanco, Alexandra (9)
|
FGASC |
1:01.54 |
1:02.18 |
0.64 |
| 19 |
Cuevas, Zoe A (9)
|
FGAJSC |
|
1:02.19 |
- |
| 20 |
Abijo, Sofi O (8)
|
FGEA |
|
1:03.55 |
- |
| 21 |
Dorta, Simone D (8)
|
FGAJSC |
|
1:20.86 |
- |