| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Morales, Angelika M (10)
|
FGEA |
42.38 |
39.41 |
-2.97 |
| 2 |
Cancio Jorge, Alma N (8)
|
FGMACM |
45.57 |
41.55 |
-4.02 |
| 3 |
Nunez-Estrada, Estefania (10)
|
UNAT |
1:07.63 |
42.26 |
-25.37 |
| 4 |
Ospina, Ana S (9)
|
UNAT |
43.64 |
42.90 |
-0.74 |
| 5 |
Salazar, Alicia A (9)
|
FGMACM |
45.28 |
43.48 |
-1.80 |
| 6 |
Kolobova, Eva * (7)
|
FGNMST |
42.30 |
46.53 |
4.23 |
| 7 |
Fenot, Rafaelle (9)
|
FGMBS |
|
46.57 |
- |
| 8 |
Wolk, Emily S (10)
|
FGMCDA |
44.05 |
46.84 |
2.79 |
| 9 |
Lisitsyn, Elizabeth (10)
|
FGNMST |
47.85 |
47.76 |
-0.09 |
| 10 |
Rincon, Maria P (10)
|
FGRANS |
|
47.86 |
- |
| 11 |
Ramos, Jenica I (9)
|
FGMACM |
26.25 |
48.04 |
21.79 |
| 12 |
Van Mossel, Emma M (10)
|
FGOSC |
46.53 |
48.86 |
2.33 |
| 13 |
Cuevas, Zoe A (9)
|
FGAJSC |
|
50.19 |
- |
| 14 |
Kotz-Agramonte, Sofia O (9)
|
FGRANS |
50.30 |
50.32 |
0.02 |
| 15 |
David, Antonia (9)
|
FGEA |
52.12 |
51.55 |
-0.57 |
| 16 |
Grant, Faith E (9)
|
FGAJSC |
51.74 |
52.31 |
0.57 |
| 17 |
Dennis, Janiya M (10)
|
FGFLA |
56.78 |
52.66 |
-4.12 |
| 18 |
Alfaro, Juliet N (10)
|
FGEA |
38.70 |
53.04 |
14.34 |
| 19 |
Blanco, Alexandra (9)
|
FGASC |
|
54.21 |
- |
| 20 |
Diaz, Maya (10)
|
FGASC |
53.48 |
55.19 |
1.71 |
| 21 |
Albuja, Rebecca M (9)
|
FGEA |
|
55.52 |
- |
| 22 |
De Angulo, Sofia G (7)
|
FGMS |
|
56.29 |
- |
| 23 |
Barreto, Zoe (7)
|
FGOSC |
1:02.92 |
57.52 |
-5.40 |
| 24 |
Candelaria, Gabriela E (9)
|
FGMACM |
53.77 |
58.20 |
4.43 |
| 25 |
Quinones, Abigail (9)
|
FGASC |
|
1:02.67 |
- |
| 26 |
Spencer-Funchion, Dorothy D (8)
|
FGCANE |
57.79 |
1:05.66 |
7.87 |
|
Kucuk-Lake, Zoey L (9)
|
FGMBS |
57.75 |
NS |
- |
|
Bachmann, Kristen S (10)
|
UNAT |
47.15 |
NS |
- |