| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Deluna, Gabriella S (12)
|
FGMWA |
35.54 |
35.02 |
-0.52 |
| 2 |
Idler, Sahara (11)
|
FGMACM |
36.80 |
36.33 |
-0.47 |
| 3 |
Lajko, Lena (11)
|
FGSFTL |
37.97 |
36.76 |
-1.21 |
| 4 |
Morris, Dylan S (12)
|
FGSFTL |
39.32 |
37.71 |
-1.61 |
| 5 |
Guerrero, Mia S (12)
|
FGMWA |
36.93 |
37.87 |
0.94 |
| 6 |
Lazzaro, Ainnara R (11)
|
FGSDST |
40.61 |
37.95 |
-2.66 |
| 7 |
Foster, Valentina (11)
|
FGMWA |
37.98 |
38.36 |
0.38 |
| 8 |
Bicerne, Amelie (11)
|
FGMWA |
42.15 |
40.35 |
-1.80 |
| 9 |
Wong, Corina (12)
|
FGMACM |
41.87 |
40.51 |
-1.36 |
| 10 |
Ortiz, Natalia V (12)
|
FGMWA |
41.50 |
41.58 |
0.08 |
| 11 |
Heldman, Adelaide H (12)
|
FGRANS |
41.69 |
42.11 |
0.42 |
| 12 |
Barranco, Christina D (11)
|
FGGRSC |
50.44 |
44.24 |
-6.20 |
| 13 |
Alfonso, Chloe S (12)
|
FGRANS |
|
44.95 |
- |
| 14 |
Garcia, Karla (12)
|
FGSDST |
|
45.86 |
- |
| 15 |
Rhett, Katlyn E (11)
|
FGMWA |
44.45 |
46.52 |
2.07 |
| 16 |
Morales Harrison, Paloma I (11)
|
FGGRSC |
50.75 |
49.76 |
-0.99 |
| 17 |
Sherman, Benyapa C (11)
|
FGSFTL |
1:04.95 |
51.39 |
-13.56 |
| 18 |
Puentes, Nicole * (11)
|
FGGRSC |
57.22 |
51.84 |
-5.38 |
| 19 |
German, Dziah (11)
|
FGSFTL |
|
55.30 |
- |
| 20 |
Regueira, Meghan N (11)
|
FGMACM |
59.43 |
55.43 |
-4.00 |
|
Boarato, Manuela D (11)
|
FGGRSC |
45.75 |
NS |
- |