| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Idler, Sahara (11)
|
FGMACM |
32.73 |
32.46 |
-0.27 |
| 2 |
Pelayes, Mercedes (12)
|
FGSDST |
35.65 |
33.18 |
-2.47 |
| 3 |
Foster, Valentina (11)
|
FGMWA |
33.21 |
33.58 |
0.37 |
| 4 |
Mantilla, Tania (11)
|
FGMWA |
34.30 |
34.57 |
0.27 |
| 5 |
Alvarez, Valentina * (11)
|
FGMACM |
37.45 |
36.66 |
-0.79 |
| 6 |
Morris, Dylan S (12)
|
FGSFTL |
40.15 |
37.23 |
-2.92 |
| 7 |
Voet, Katherine L (11)
|
FGSFTL |
39.15 |
37.55 |
-1.60 |
| 8 |
Lazzaro, Ainnara R (11)
|
FGSDST |
38.25 |
38.06 |
-0.19 |
| 9 |
Poling, Summer R (11)
|
FGSFTL |
42.66 |
38.25 |
-4.41 |
| 10 |
Halliburton, Mercedes (12)
|
FGCANE |
44.20 |
39.11 |
-5.09 |
| 11 |
Mogul, Iris I (12)
|
FGCANE |
45.02 |
39.89 |
-5.13 |
| 12 |
Ortiz, Natalia V (12)
|
FGMWA |
44.70 |
43.60 |
-1.10 |
| 13 |
Cardenas Uribe, Catalina R (11)
|
FGGRSC |
43.44 |
43.82 |
0.38 |
| 14 |
Heldman, Adelaide H (12)
|
FGRANS |
42.14 |
45.16 |
3.02 |
| 15 |
Regueira, Meghan N (11)
|
FGMACM |
53.29 |
51.56 |
-1.73 |
| 16 |
Fininikin, D'Nadine (11)
|
FGFLA |
|
52.61 |
- |
|
Kelble, Calla N (11)
|
FGMS |
|
NS |
- |