| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Libal, Sean P (10)
|
WISWAT |
41.82 |
37.53 |
-4.29 |
| 2 |
Hernandez, Benjamin R (8)
|
WISWAT |
40.52 |
39.38 |
-1.14 |
| 3 |
Beres, Benjamin N (10)
|
WISWAT |
41.97 |
40.17 |
-1.80 |
| 4 |
Chetreanu, David A (10)
|
WIPX3 |
42.60 |
43.40 |
0.80 |
| 5 |
Ward-Wade, Colton J (10)
|
WISWAT |
51.08 |
51.29 |
0.21 |
| 6 |
Race, Nathan M (9)
|
WISWAT |
47.33 |
51.42 |
4.09 |
| 7 |
Agger, Dane D (9)
|
WISWAT |
50.45 |
51.54 |
1.09 |
| 7 |
Malinis, Sean Riley J (10)
|
WISWAT |
53.57 |
51.54 |
-2.03 |
| 9 |
Volm, Colin J (10)
|
WILGSC |
|
53.06 |
- |
| 10 |
Burzynski, Braden J (9)
|
WISWAT |
1:00.74 |
58.07 |
-2.67 |
| 11 |
Salbreiter, Jacob D (9)
|
WISWAT |
56.37 |
58.84 |
2.47 |
|
Martin, Keegan P (10)
|
WIPX3 |
|
DQ |
- |
|
Krebs, Nolan D (10)
|
WITOSA |
|
DQ |
- |