| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Miller, Sydney E (15)
|
WISWAT |
1:02.04 |
1:01.38 |
-0.66 |
| 2 |
Tselemegkou, Nefeli (14)
|
WISSTY |
|
1:01.84 |
- |
| 3 |
Jankowski, Madison K (15)
|
WISWAT |
1:06.29 |
1:02.46 |
-3.83 |
| 4 |
Cortright, Lauren J (16)
|
WITOSA |
1:02.41 |
1:04.67 |
2.26 |
| 4 |
Gabor, Estella S (15)
|
WISSTY |
1:03.88 |
1:04.67 |
0.79 |
| 6 |
Paquette, Julia L (16)
|
WISSTY |
1:03.69 |
1:04.98 |
1.29 |
| 7 |
Hebert, Rebecca B (16)
|
WISWAT |
1:09.22 |
1:05.48 |
-3.74 |
| 8 |
Herzog, Maya K (16)
|
WIPX3 |
1:05.58 |
1:05.98 |
0.40 |
| 9 |
O'Brien, Lauren R (16)
|
WILGSC |
1:03.94 |
1:06.05 |
2.11 |
| 10 |
Minahan, Isabella A (15)
|
WITOSA |
1:11.43 |
1:06.21 |
-5.22 |
| 11 |
Wallander, Alyssa (15)
|
WIPX3 |
1:07.50 |
1:06.36 |
-1.14 |
| 12 |
Davis, Olivia A (16)
|
WISWAT |
1:04.96 |
1:06.40 |
1.44 |
| 13 |
Krajnak, Margaret A (15)
|
WISWAT |
1:13.15 |
1:06.57 |
-6.58 |
| 14 |
Marquez, Sara (16)
|
WISWAT |
1:05.66 |
1:06.73 |
1.07 |
| 15 |
Reischl, Mackenzie J (16)
|
WISEA |
1:08.58 |
1:06.84 |
-1.74 |
| 16 |
Ceshker, Madison A (15)
|
WILGSC |
1:10.46 |
1:07.15 |
-3.31 |
| 17 |
Scharff, Alison M (17)
|
WISWAT |
1:04.15 |
1:07.32 |
3.17 |
| 18 |
Cady, Meghan O (11)
|
WITOSA |
1:08.61 |
1:07.45 |
-1.16 |
| 19 |
Mellock, Megan L (15)
|
WISWAT |
1:11.61 |
1:07.95 |
-3.66 |
| 20 |
Wember, Eden R (17)
|
WIGYD |
1:05.42 |
1:08.58 |
3.16 |
| 21 |
Weinstock, Megan R (16)
|
WILGSC |
1:10.62 |
1:08.65 |
-1.97 |
| 22 |
Bartz, Madeline A (15)
|
WITOSA |
1:09.66 |
1:10.49 |
0.83 |
| 23 |
McElvain, Delaney C (15)
|
WISWAT |
1:13.43 |
1:12.10 |
-1.33 |
| 24 |
Kobus, Kelsey L (15)
|
WISWAT |
1:17.97 |
1:12.20 |
-5.77 |
| 25 |
Riviere, Collette I (15)
|
WILGSC |
1:12.99 |
1:14.64 |
1.65 |
| 26 |
Stratman, Elizabeth R (15)
|
WISEA |
1:19.28 |
1:20.41 |
1.13 |