| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Walker, Lucy E (10)
|
MRSS |
43.12 |
40.08 |
-3.04 |
| 2 |
Schlifkin, Marissa (10)
|
MRMWSC |
42.73 |
42.10 |
-0.63 |
| 3 |
Ruiz, Anaya (10)
|
MRUN |
|
45.61 |
- |
| 4 |
Psilopoulos, Andie J (10)
|
ADHAWK |
46.58 |
46.77 |
0.19 |
| 5 |
Gatterdam, Lyla (10)
|
MRSS |
50.70 |
46.78 |
-3.92 |
| 6 |
Pearsall, Eloise M (10)
|
MRPATS |
46.39 |
47.13 |
0.74 |
| 7 |
Parasnis, Gabriella A (10)
|
MRSS |
54.73 |
47.15 |
-7.58 |
| 8 |
Burdick, Riley E (10)
|
ADHAWK |
47.77 |
48.53 |
0.76 |
| 9 |
Pece, Delaney R (9)
|
ADHAWK |
49.68 |
48.75 |
-0.93 |
| 10 |
Gash, Amelia M (9)
|
MRPATS |
48.47 |
50.15 |
1.68 |
| 11 |
Lischinsky, Claire M (10)
|
ADHAWK |
51.20 |
50.74 |
-0.46 |
| 12 |
Phillips, Leela M (10)
|
MRSS |
50.11 |
50.81 |
0.70 |
| 13 |
Panaro, Sadie A (9)
|
MRPATS |
51.14 |
51.84 |
0.70 |
| 14 |
Mischka, Camilla E (10)
|
MRSS |
55.31 |
51.99 |
-3.32 |
| 15 |
Friedman, Alexa L (10)
|
ADHAWK |
1:20.00 |
56.89 |
-23.11 |
| 16 |
Lockward, Rebeca P (10)
|
MRYBAR |
56.83 |
57.81 |
0.98 |
| 17 |
Pino, Juliana T (9)
|
MRDA |
1:00.21 |
1:02.26 |
2.05 |
| 18 |
Fortuna, Aoife C (10)
|
MRPATS |
|
1:02.67 |
- |
| 19 |
Reyes, Marina L (9)
|
MRNDAC |
1:09.15 |
1:04.58 |
-4.57 |
| 20 |
Loyer, Sofia A (10)
|
ADHAWK |
1:19.60 |
1:04.98 |
-14.62 |
| 21 |
Zaucha, Bianca A (9)
|
MRUN |
1:17.95 |
1:13.07 |
-4.88 |
|
Vos, Brooke C (9)
|
ADHAWK |
1:05.27 |
DQ |
- |
|
Ermer, Freja E (9)
|
ADHAWK |
1:20.49 |
NS |
- |