| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bao, Hanyu (10)
|
MRNYSA |
33.46 |
36.00 |
2.54 |
| 2 |
Briceno, Isabella (9)
|
MRNYSA |
36.07 |
38.86 |
2.79 |
| 3 |
Giannini, Alanah M (10)
|
MRPBAC |
39.64 |
38.91 |
-0.73 |
| 4 |
Buliung, Madison V (10)
|
MRAGMV |
48.55 |
44.43 |
-4.12 |
| 5 |
McCarron, Olivia S (10)
|
MRTRS |
|
46.43 |
- |
| 6 |
Cornine, Mary Catherine A (10)
|
MRYBAR |
48.59 |
51.37 |
2.78 |
| 7 |
Sheldon, eva I (10)
|
MRNYSA |
52.14 |
54.02 |
1.88 |
| 8 |
Messing, Samantha K (9)
|
MRVAC |
55.02 |
56.23 |
1.21 |
| 9 |
O'Brien, Abigail C (9)
|
MRAGMV |
1:21.12 |
1:10.65 |
-10.47 |
|
Chakrabarti, Arya Ocean (10)
|
MRTRS |
|
DQ |
- |