| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thompson, Samantha K (10)
|
MRVAC |
49.29 |
45.38 |
-3.91 |
| 2 |
Jones-Torres, Olivia-Ziva J (10)
|
MRPBAC |
55.38 |
47.54 |
-7.84 |
| 3 |
Gagnon, Maryann T (10)
|
MRPAC |
NT |
49.78 |
49.78 |
| 4 |
Messing, Samantha K (10)
|
MRVAC |
53.12 |
50.62 |
-2.50 |
| 5 |
Hajek, Anna L (9)
|
MRPBAC |
59.11 |
50.75 |
-8.36 |
| 6 |
Boyle, Dakota R (9)
|
MRHVD |
52.81 |
50.77 |
-2.04 |
| 7 |
Haydt, Abigail G (10)
|
MRPATS |
52.07 |
51.63 |
-0.44 |
| 8 |
Donikyan, Adrina A (9)
|
MRPATS |
51.56 |
52.63 |
1.07 |
| 9 |
Ayala, Sophia R (10)
|
MRVAC |
51.73 |
53.04 |
1.31 |
| 10 |
Nemeth, Addison C (10)
|
MRHVD |
56.29 |
54.24 |
-2.05 |
| 11 |
Abulencia, Michaela E (10)
|
MRPATS |
54.05 |
55.71 |
1.66 |
| 12 |
Pennock, Lyris A (9)
|
MRHVD |
1:07.56 |
59.66 |
-7.90 |
| 13 |
Newberns, Maya E (10)
|
MRPBAC |
1:04.63 |
1:01.12 |
-3.51 |
| 14 |
Hundley, Della A (8)
|
MRPBAC |
1:22.23 |
1:04.95 |
-17.28 |
| 15 |
Zagon, Emersyn W (9)
|
MRPAC |
NT |
1:06.23 |
66.23 |
| 16 |
Xu, Eleanor Z (8)
|
MRPATS |
1:17.91 |
1:08.03 |
-9.88 |
| 17 |
Ayuso, Brielle (9)
|
MRPAC |
NT |
DQ |
- |
| 18 |
Doherty, Madeleine R (9)
|
MRHVD |
NT |
DQ |
- |
| 19 |
Krasikova, Ania (6)
|
MRPATS |
NT |
DQ |
- |
| 20 |
Torres, Valeria A (9)
|
MRPAC |
NT |
DQ |
- |
| 21 |
McGuinness, Maura J (9)
|
MRPATS |
NT |
DQ |
- |