| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Amaro, Dylan J (9)
|
MRNDAC |
41.33 |
41.94 |
0.61 |
| 2 |
Singhal, Tanmay (10)
|
MRSPAR |
44.55 |
43.45 |
-1.10 |
| 3 |
Herbert, Owain G (10)
|
MRNDAC |
48.97 |
46.39 |
-2.58 |
| 4 |
Tolis, Alexander B (9)
|
MRAG |
45.59 |
46.73 |
1.14 |
| 5 |
Leahy, Christopher J (10)
|
MRVAC |
|
50.04 |
- |
| 6 |
Storaska, Nathaniel W (10)
|
MRVAC |
|
51.08 |
- |
| 7 |
Taranto, John T (10)
|
MRYBAR |
52.36 |
54.69 |
2.33 |
| 8 |
Albanese, Luke W (9)
|
MRYBAR |
55.28 |
54.82 |
-0.46 |
| 9 |
Arevalo, Brayan A (10)
|
MRSPAR |
57.52 |
56.66 |
-0.86 |
| 10 |
Elmassalemah, Iad K (10)
|
MRNDAC |
57.29 |
59.23 |
1.94 |
| 11 |
Luna, Gabriel (10)
|
MRWSSC |
58.67 |
1:04.88 |
6.21 |
| 12 |
Vergara, Alexander (9)
|
MRSPAR |
1:04.98 |
1:09.73 |
4.75 |
|
Barnett, Jai (9)
|
MRVAC |
|
DQ |
- |
|
Babcock, Bryan (10)
|
MRWSSC |
1:10.84 |
DQ |
- |