| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
O'Brien, Connor S (12)
|
MRBGNW |
28.33 |
28.72 |
0.39 |
| 2 |
Tchernikov, Phillip A (12)
|
MRCOND |
28.68 |
28.76 |
0.08 |
| 3 |
Lazatin, Thomas J (12)
|
MRVAC |
28.96 |
28.78 |
-0.18 |
| 4 |
Lynaugh, Matthew L (12)
|
MRMWDA |
29.23 |
29.09 |
-0.14 |
| 5 |
Cinek, Zachary (12)
|
MRPAC |
28.88 |
29.18 |
0.30 |
| 6 |
Battaglia, Matthew E (12)
|
MRBGNW |
29.77 |
29.19 |
-0.58 |
| 7 |
Abdel-Kader, Anthony P (12)
|
MRMWDA |
30.18 |
29.66 |
-0.52 |
| 8 |
Cohen, Hayden S (12)
|
MRRA |
28.06 |
29.83 |
1.77 |
| 9 |
Rice, Andrew D (12)
|
MRBGNW |
29.16 |
29.89 |
0.73 |
| 10 |
Bartolacci, Matthew M (12)
|
MRRA |
29.80 |
30.02 |
0.22 |
| 11 |
Kumar, Aadi (12)
|
MRYMID |
30.45 |
30.29 |
-0.16 |
| 12 |
Tennant, Jackson M (12)
|
MRNBS |
30.07 |
30.40 |
0.33 |
|
Rusiecki, Daniel P (12)
|
MRNYS |
27.57 |
27.09 |
-0.48 |
|
Townsend, Lukas J (12)
|
MRNBS |
28.57 |
27.79 |
-0.78 |
|
North, Benjamin D (12)
|
MRHVD |
30.60 |
DQ |
- |