| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Joachim, Gianna M (8)
|
MRNBS |
42.63 |
39.90 |
-2.73 |
| 2 |
Kuzicki, Isabella R (8)
|
MRAGMV |
42.13 |
41.71 |
-0.42 |
| 3 |
Bao, Hanyin (7)
|
MRNYSA |
51.57 |
46.64 |
-4.93 |
| 4 |
Afanasewicz, Kate N (8)
|
MRNYSA |
48.06 |
49.65 |
1.59 |
| 5 |
Butler, Mikayla S (8)
|
MRWSSC |
1:06.69 |
53.91 |
-12.78 |
| 6 |
Touijar, Zineb (7)
|
MRMHHW |
55.03 |
54.74 |
-0.29 |
| 7 |
Martinez Santos, Meyali (8)
|
MRMHHW |
55.20 |
58.50 |
3.30 |
| 8 |
Kuzicki, Kendra J (7)
|
MRAGMV |
55.10 |
59.32 |
4.22 |
| 9 |
Figueroa, Ximena (6)
|
MRMHHW |
56.03 |
59.55 |
3.52 |
| 10 |
Pucino, Krista (8)
|
MRNBS |
1:05.29 |
1:01.71 |
-3.58 |
| 11 |
Cabrera-Zavala, Allison (7)
|
MRMHHW |
1:00.81 |
1:02.36 |
1.55 |
| 12 |
Sarnicki, Brielle A (7)
|
MRAGMV |
1:05.63 |
1:06.81 |
1.18 |
| 13 |
Boyce, Molly B (8)
|
MRVAC |
1:02.06 |
1:08.26 |
6.20 |
| 14 |
Carrigan, Kaylee (7)
|
MRRA |
1:03.37 |
1:09.01 |
5.64 |
| 15 |
Pazoga, Sophia (6)
|
MRVAC |
1:09.46 |
1:20.18 |
10.72 |
| 16 |
Xin, Violet Z (6)
|
MRAGMV |
1:45.89 |
1:23.45 |
-22.44 |