| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tan, Samantha N (15)
|
MRBGNW |
25.30 |
25.72 |
0.42 |
| 2 |
Meyerhoeffer, Stella M (15)
|
MRYMID |
25.70 |
25.80 |
0.10 |
| 3 |
Rocco, Emily A (15)
|
MRBGNW |
25.31 |
27.12 |
1.81 |
| 4 |
Shopovick, Sophia F (15)
|
MRYMID |
26.34 |
27.36 |
1.02 |
| 5 |
Zhu, Catherine (17)
|
MRBGNW |
26.74 |
27.57 |
0.83 |
| 6 |
Kopasz, Nicole (15)
|
MRYMID |
27.92 |
28.01 |
0.09 |
| 6 |
Gualtiere, Isabella K (16)
|
MRUN |
27.39 |
28.01 |
0.62 |
| 8 |
Desiderio, Sarah P (15)
|
MRBGNW |
27.74 |
28.47 |
0.73 |
| 9 |
Garofolo, Brianna B (15)
|
MRBGNW |
26.70 |
29.31 |
2.61 |
| 10 |
Smith, Caroline K (15)
|
MRYMID |
29.13 |
29.41 |
0.28 |
| 11 |
Ford, Sydney E (17)
|
MRBGNW |
27.68 |
29.54 |
1.86 |
| 12 |
Byrd, Christine E (16)
|
MRYMID |
28.89 |
29.70 |
0.81 |
| 13 |
Laurenceau, Alyssa M (16)
|
MRYMID |
28.99 |
30.53 |
1.54 |
| 14 |
Greenstein, Jessie B (15)
|
MRBGNW |
29.68 |
30.73 |
1.05 |
| 14 |
Hallac, Anna C (15)
|
MRBGNW |
29.43 |
30.73 |
1.30 |
|
Belmar, Ayanna F (17)
|
MRYMID |
29.32 |
NS |
- |