| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Waggoner, Gabriel J (7)
|
MAJW |
26.81 |
26.90 |
0.09 |
| 2 |
Masucci, Thomas L (7)
|
MAVINY |
29.96 |
28.89 |
-1.07 |
| 3 |
Kerins, Dylan S (7)
|
MATRI |
29.46 |
29.32 |
-0.14 |
| 4 |
Kerins, Gavin T (7)
|
MATRI |
33.40 |
30.77 |
-2.63 |
| 5 |
McMillan, Ryan S (7)
|
MAJW |
|
31.53 |
- |
| 6 |
Papadopoulos, Fotios S (7)
|
MATRI |
39.15 |
34.68 |
-4.47 |
| 7 |
Walsh, Cillian J (7)
|
MAJW |
38.73 |
35.17 |
-3.56 |
| 8 |
Lam, Connor T (7)
|
MAJW |
43.27 |
37.56 |
-5.71 |
| 9 |
Chon, Aiden L (7)
|
MAJW |
|
39.46 |
- |
| 10 |
Shields, Rylan D (7)
|
MAJW |
48.55 |
48.72 |
0.17 |
|
Aum, Tate K (7)
|
MAFSA |
36.49 |
NS |
- |
|
Heebner-Elliott, Trevor W (7)
|
MAGRA |
|
NS |
- |