| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sanders, Lily G (9)
|
PVRMSC |
42.64 |
40.95 |
-1.69 |
| 2 |
Crown, Addison A (9)
|
PVRMSC |
43.08 |
41.27 |
-1.81 |
| 3 |
Lee, Olivia M (9)
|
PVRMSC |
43.18 |
44.18 |
1.00 |
| 4 |
He, Elizabeth (9)
|
PVRMSC |
45.05 |
45.89 |
0.84 |
| 5 |
Jayaprakash, Tanmaya (9)
|
PVRMSC |
|
47.68 |
- |
| 6 |
Fry, Julianne M (9)
|
PVRMSC |
|
47.75 |
- |
| 7 |
Nowak, Anna G (9)
|
PVDCPR |
54.17 |
50.24 |
-3.93 |
| 8 |
Purdy, Emerson N (9)
|
PVRMSC |
52.86 |
51.30 |
-1.56 |
| 9 |
Cypes, Sloane B (9)
|
PVASA |
46.50 |
51.36 |
4.86 |
| 10 |
Shen, Melissa (9)
|
PVRMSC |
53.74 |
51.61 |
-2.13 |
| 11 |
Burke-Chinarro, Isabel (9)
|
PVDCPR |
58.24 |
58.59 |
0.35 |
| 12 |
Anyabuike, Anuli N (9)
|
PVDCPR |
|
59.77 |
- |
| 13 |
Carballo-Naranjo, Reina D (9)
|
PVDCPR |
1:10.38 |
1:03.51 |
-6.87 |
|
Black, June R (9)
|
PVDCPR |
1:27.57 |
NS |
- |