| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Curtis, Grace E (10)
|
MASJAC |
39.13 |
40.01 |
0.88 |
| 2 |
Albuquerque, Sylvia G (10)
|
MASJAC |
50.90 |
43.86 |
-7.04 |
| 3 |
Royal, Megan J (10)
|
MASJAC |
|
44.67 |
- |
| 4 |
Torres-Thorpe, Charlie A (10)
|
MASJAC |
46.49 |
45.91 |
-0.58 |
| 5 |
Merch, Laura A (9)
|
MASJAC |
51.32 |
47.42 |
-3.90 |
| 6 |
Davis, Kennedy N (10)
|
MASJAC |
49.27 |
47.54 |
-1.73 |
| 7 |
McMahon, Bridget K (10)
|
MAUNSJ |
|
48.13 |
- |
| 8 |
Ruggieri, Kyllie (10)
|
MASJAC |
|
48.38 |
- |
| 9 |
DeNucci, Francesca T (10)
|
MASJAC |
50.49 |
48.96 |
-1.53 |
| 10 |
Scott, Emma G (10)
|
MASJAC |
52.42 |
49.31 |
-3.11 |
| 11 |
Kiesel, Addison E (9)
|
MASJAC |
58.00 |
50.69 |
-7.31 |
| 12 |
Guerrini, Gabriella R (9)
|
MAUNSJ |
|
53.77 |
- |
| 13 |
Fleming, Ainsley H (9)
|
MASJAC |
|
55.12 |
- |
| 14 |
Corrielus, Jayna A (9)
|
MASJAC |
|
56.02 |
- |
| 15 |
Mehta, Norah R (10)
|
MASJAC |
57.04 |
58.37 |
1.33 |
| 16 |
Mullin, Kiera S (9)
|
MASJAC |
|
1:04.40 |
- |
| 17 |
Betancourt, Camila R (10)
|
MASJAC |
|
1:06.75 |
- |
|
Overton, Madison (9)
|
MASJAC |
|
DQ |
- |
|
Kouser, Kaitlyn M (10)
|
MASJAC |
49.38 |
NS |
- |
|
Anton, Lauren A (10)
|
MASJAC |
43.34 |
NS |
- |