| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nie, Kathy Y (12)
|
SRBY |
32.98 |
34.36 |
1.38 |
| 2 |
Tognoni, Isabella M (11)
|
SRSV5B |
36.13 |
35.18 |
-0.95 |
| 3 |
Schaffer, Sadie S (12)
|
SRNST |
37.01 |
35.87 |
-1.14 |
| 4 |
Dimitriu, Mara M (11)
|
SRBSC |
37.63 |
37.66 |
0.03 |
| 5 |
Pickett, Sienna C (12)
|
SRBY |
|
42.95 |
- |
| 6 |
Kerr, Reese M (12)
|
SRBY |
|
43.10 |
- |
| 7 |
Gisslen, Annaliese J (12)
|
SRBSC |
45.36 |
44.41 |
-0.95 |
| 8 |
Mutchie, Kallen D (12)
|
SRBY |
50.73 |
46.62 |
-4.11 |
| 9 |
Flores, Eliana O (11)
|
SRBY |
53.81 |
48.23 |
-5.58 |
| 10 |
McShane, Tavianne E (11)
|
SRNST |
47.96 |
48.72 |
0.76 |
| 11 |
Campbell, Rebekah N (12)
|
SRSA |
50.69 |
50.35 |
-0.34 |
| 12 |
Mueller, Anja I (12)
|
SRBY |
|
50.68 |
- |
| 13 |
Barnett, Genavive D (11)
|
SRNST |
56.51 |
52.57 |
-3.94 |
| 14 |
Brewster, Campbell R (11)
|
SRBY |
|
54.81 |
- |
| 16 |
Poggi, Evelyn R (11)
|
SRSA |
|
55.71 |
- |
| 17 |
Wensel, Helen M (11)
|
SRBY |
59.59 |
1:01.20 |
1.61 |
| 18 |
Vasso, Maya K (11)
|
SRNST |
1:03.89 |
1:01.64 |
-2.25 |
| 19 |
Gray, Nevaeh A (11)
|
SREST |
|
1:05.83 |
- |
|
Arce, Olivia C (11)
|
SRBY |
|
DQ |
- |