| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Karasinski, Gabriella (8)
|
MAPENN |
38.78 |
38.37 |
-0.41 |
| 2 |
Lebedinskiy, Chelsea N (8)
|
MATHY |
40.93 |
39.08 |
-1.85 |
| 3 |
Gallagher, Brynn (8)
|
MATVAC |
41.16 |
39.43 |
-1.73 |
| 4 |
Scibona, Ellie J (8)
|
MATHY |
41.06 |
40.89 |
-0.17 |
| 5 |
McPeek, Kelsey M (8)
|
MAPSC |
44.01 |
41.88 |
-2.13 |
| 6 |
Kehoe-Huck, Adelaide N (8)
|
MAPSC |
41.99 |
41.96 |
-0.03 |
| 7 |
Teichman, Alexis L (8)
|
MAEMAC |
45.37 |
43.61 |
-1.76 |
| 8 |
Kane, Victoria G (8)
|
MATVAC |
46.73 |
44.19 |
-2.54 |
| 9 |
Hower, Megan J (8)
|
MAUN-T |
|
44.39 |
- |
| 10 |
Evans, Emily (8)
|
MATVAC |
46.33 |
45.01 |
-1.32 |
| 11 |
Loebsack, Sydney A (8)
|
MABLUE |
57.31 |
46.57 |
-10.74 |
| 12 |
Heiserman, Lindsey R (8)
|
MALVA |
1:00.08 |
52.73 |
-7.35 |
| 13 |
Shannon, Lia M (8)
|
MABLUE |
1:03.40 |
1:00.47 |
-2.93 |
| 14 |
Buglino, Kaylee A (8)
|
MALVA |
1:04.51 |
1:01.27 |
-3.24 |
|
Davison, Olivia P (8)
|
MABLUE |
|
NS |
- |