| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bacinski, Riley (8)
|
NJHQH |
42.78 |
38.61 |
-4.17 |
| 2 |
McGlynn, Shea (8)
|
MAPFY |
41.66 |
41.36 |
-0.30 |
| 3 |
Nalli, Alexia C (8)
|
MABLUE |
41.17 |
41.70 |
0.53 |
| 4 |
Pappentick, Addison K (8)
|
MAEMAC |
41.01 |
41.95 |
0.94 |
| 5 |
Cielecki, Jessica (8)
|
MAAAC |
46.50 |
43.58 |
-2.92 |
| 6 |
Farag, Hanna (8)
|
MAEAST |
42.92 |
45.14 |
2.22 |
| 7 |
Weathers, Layla S (8)
|
MAEAST |
1:10.77 |
45.71 |
-25.06 |
| 8 |
Fry, Emma G (8)
|
MABLUE |
|
45.90 |
- |
| 9 |
Ragab, Alexia S (8)
|
MABLUE |
58.16 |
50.68 |
-7.48 |
| 10 |
Sciarretta, Natalie M (8)
|
MATVAC |
|
51.74 |
- |
| 11 |
Kachala, Sienna R (8)
|
MABLUE |
|
53.59 |
- |
| 12 |
Ismail, Salma A (8)
|
MABLUE |
59.67 |
55.43 |
-4.24 |
| 13 |
Wallace, Avery L (8)
|
MAEAST |
52.64 |
56.89 |
4.25 |
|
Horace, Caroline G (8)
|
MAAAC |
53.06 |
NS |
- |