| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Henkel, Brayden D (12)
|
ADALBS |
33.88 |
32.77 |
-1.11 |
| 2 |
Wilson, Edmund (12)
|
MRNDAC |
36.24 |
35.43 |
-0.81 |
| 3 |
Brkanovic, Isak A (11)
|
ADDELM |
38.57 |
39.44 |
0.87 |
| 4 |
Abramson, Jesse A (12)
|
MRAGMV |
1:10.89 |
42.20 |
-28.69 |
| 5 |
Bulumulla, Vidhu D (12)
|
ADALBS |
1:01.40 |
42.89 |
-18.51 |
| 6 |
Majumdar, Ryan (11)
|
MRHVD |
42.50 |
43.11 |
0.61 |
| 7 |
LaFountain, Tobias P (12)
|
ADALBS |
43.69 |
44.32 |
0.63 |
| 8 |
Lischinsky, Stephen J (11)
|
ADHAWK |
42.28 |
44.81 |
2.53 |
| 9 |
Cerrato, Jacob A (11)
|
MRMWDA |
42.98 |
45.16 |
2.18 |
| 10 |
Johnson, William F (12)
|
MRHVD |
43.82 |
45.33 |
1.51 |
| 11 |
Dorner, Benjamin (11)
|
MRNDAC |
43.97 |
45.37 |
1.40 |
| 12 |
Burgarelli, Sebastian (11)
|
MRNBS |
47.58 |
49.00 |
1.42 |
| 13 |
Bhalla, Jack S (12)
|
ADHAWK |
|
52.21 |
- |
| 14 |
Mummery, John F (11)
|
MRNBS |
54.77 |
57.04 |
2.27 |
|
Kuang, Everest Z (11)
|
ADALBS |
|
NS |
- |
|
Fairbanks, Gage L (12)
|
MRNBS |
49.27 |
NS |
- |