| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Crawford, Averie M (10)
|
MADST |
40.19 |
38.98 |
-1.21 |
| 1 |
McKeon, Kasidy B (10)
|
MAECCC |
45.02 |
41.68 |
-3.34 |
| 1 |
Edge, Olivia Q (9)
|
MAGNBC |
52.93 |
45.60 |
-7.33 |
| 2 |
Rykaczewski, Reagan E (10)
|
MADST |
49.40 |
45.70 |
-3.70 |
| 2 |
Kilyk, Miranda (10)
|
MAMUHL |
47.03 |
45.99 |
-1.04 |
| 3 |
Chambers, Jenna B (9)
|
MAMAKO |
44.41 |
46.08 |
1.67 |
| 3 |
Iliadis, Savina (10)
|
MADST |
48.84 |
46.15 |
-2.69 |
| 4 |
Gottesman, Serena D (10)
|
MADST |
51.03 |
47.14 |
-3.89 |
| 5 |
King, Madison K (10)
|
MAGNBC |
54.38 |
47.41 |
-6.97 |
| 6 |
Wang, Kayla (9)
|
MADST |
58.90 |
51.75 |
-7.15 |
| 7 |
Pan, Sophie Y (9)
|
MADST |
1:00.34 |
52.24 |
-8.10 |
| 8 |
Minter, Paisley F (9)
|
MADST |
54.66 |
52.30 |
-2.36 |
| 9 |
Gunther, Ella M (9)
|
MADST |
54.96 |
52.61 |
-2.35 |
| 10 |
Kotowski, Kaitlyn G (10)
|
MAGNBC |
54.25 |
52.99 |
-1.26 |
| 11 |
Schroeder, Caroline K (9)
|
MAECCC |
52.91 |
54.38 |
1.47 |
| 12 |
Collins, Lexi A (9)
|
MAMAKO |
|
55.78 |
- |
| 13 |
Whalen, Charlotte M (9)
|
MAECCC |
1:07.51 |
56.93 |
-10.58 |
| 14 |
Barnett, Emily R (10)
|
MADST |
|
57.11 |
- |
| 15 |
Texter, Sarah M (9)
|
MAECCC |
1:11.92 |
1:00.51 |
-11.41 |
| 16 |
Bradley, Lillie M (9)
|
MAMAKO |
1:11.91 |
1:01.04 |
-10.87 |
| 17 |
Kulon, Matylda N (10)
|
MADST |
|
1:02.21 |
- |
| 18 |
Lutes, Delaney O (9)
|
MADST |
1:04.56 |
1:02.35 |
-2.21 |
| 19 |
Carlisle, Lilly J (10)
|
MAMAKO |
1:17.56 |
1:06.71 |
-10.85 |