| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Troy, Kaitlyn E (17)
|
MRNBS |
5:23.61 |
5:20.12 |
-3.49 |
| 2 |
Cullinan, Madison (16)
|
MRNYSA |
5:23.17 |
5:33.25 |
10.08 |
| 3 |
Regenauer, Christiana M (13)
|
MRDA |
|
5:34.63 |
- |
| 4 |
Bacigal, Paige M (13)
|
MRNBS |
5:49.65 |
5:35.82 |
-13.83 |
| 5 |
Frey, Sophie G (16)
|
MRNYSA |
5:33.75 |
5:38.69 |
4.94 |
| 6 |
Ayala, Ashley N (13)
|
MRUNNB |
5:57.07 |
5:48.30 |
-8.77 |
| 7 |
Bosse, Clare M (13)
|
MRNBS |
5:52.18 |
5:51.96 |
-0.22 |
| 8 |
Morgan, Alice T (16)
|
MRNBS |
5:51.69 |
5:56.11 |
4.42 |
| 9 |
Noble, Allison E (15)
|
MRHVD |
5:44.88 |
5:56.46 |
11.58 |
| 10 |
Vela, Allison J (14)
|
MRNBS |
6:30.15 |
6:00.27 |
-29.88 |
| 11 |
Beneat, Lauren E (14)
|
MRMWSC |
5:36.46 |
6:02.08 |
25.62 |
| 12 |
Regenauer, Emma A (17)
|
MRDA |
5:55.01 |
6:12.10 |
17.09 |
| 13 |
DeBenedictus, Kaylee R (16)
|
MRNBS |
5:54.91 |
6:16.91 |
22.00 |
| 14 |
Seward, Kristen J (15)
|
MRHVD |
7:11.98 |
6:16.96 |
-55.02 |
| 15 |
Lopez, Iliana L (13)
|
MRNBS |
7:14.54 |
6:27.83 |
-46.71 |
| 16 |
Reede, Sydney A (13)
|
MRNBS |
6:51.46 |
6:28.23 |
-23.23 |
| 17 |
Jarvis, MacKenzie T (15)
|
MRHVD |
6:49.27 |
6:37.90 |
-11.37 |
| 18 |
Quade, Sarah N (17)
|
MRMVA |
6:40.30 |
6:42.34 |
2.04 |
| 19 |
Dewhurst, Julia J (12)
|
MRDA |
6:40.95 |
6:57.39 |
16.44 |
| 20 |
Pietraszka, Sarah G (14)
|
MRMVA |
6:49.12 |
6:59.64 |
10.52 |