| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Davis, Mckennly (9)
|
ZZCRU |
|
44.69 |
- |
| 2 |
Nance, Madelyn P (9)
|
VASEVA |
52.96 |
50.33 |
-2.63 |
| 3 |
Sarinas, Mara-Alexandria C (9)
|
VACGBD |
|
50.69 |
- |
| 4 |
Bellflower, Mary Catherine (9)
|
ZZCRU |
|
51.62 |
- |
| 5 |
Narkawicz, Elizabeth (10)
|
ZZCRU |
|
53.59 |
- |
| 6 |
Senterfit, Abby (9)
|
ZZCRU |
|
54.21 |
- |
| 7 |
Townsend, Sophia G (9)
|
VACGBD |
1:00.69 |
56.76 |
-3.93 |
| 8 |
Simeral, Delaney K (10)
|
VACGBD |
1:16.00 |
59.15 |
-16.85 |
| 9 |
Elsaadat, Maya (9)
|
ZZCRU |
58.00 |
1:01.53 |
3.53 |
| 10 |
Leffel, Vera (9)
|
ZZCRU |
59.80 |
1:05.16 |
5.36 |
| 11 |
Bliss, Ayla (9)
|
ZZCRU |
1:02.50 |
1:10.65 |
8.15 |
|
Tomblin, Heather A (9)
|
VACGBD |
58.06 |
NS |
- |