| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reber, Peyton E (6)
|
MAFSSC |
24.42 |
25.09 |
0.67 |
| 2 |
Klaehn, Milla L (6)
|
MATRI |
25.53 |
26.61 |
1.08 |
| 3 |
Stewart, Quinlan M (6)
|
MAWAP |
27.21 |
28.45 |
1.24 |
| 4 |
Deveney, Brynn K (6)
|
MATRI |
31.67 |
28.56 |
-3.11 |
| 5 |
Cooper, Lindsay A (6)
|
MATRI |
30.48 |
29.24 |
-1.24 |
| 6 |
Scott, Lucy M (6)
|
MAWAC |
|
29.94 |
- |
| 7 |
Bohn, Freya A (6)
|
MAWAP |
31.91 |
31.53 |
-0.38 |
| 8 |
Jones, Madeleine H (6)
|
MAWAC |
38.84 |
33.01 |
-5.83 |
| 9 |
Jubb, Penelope E (6)
|
MAWAC |
35.71 |
33.64 |
-2.07 |
| 10 |
McWilliams, Addison G (6)
|
MAWWC |
|
35.87 |
- |
| 11 |
Bijlani, Shaina J (6)
|
MAMSA |
32.86 |
37.69 |
4.83 |
| 12 |
Grafe, Elizabeth J (6)
|
MAWAC |
36.29 |
42.14 |
5.85 |
| 13 |
Benevento, Saoirse L (6)
|
MAWAP |
45.77 |
44.55 |
-1.22 |
|
Brost, Ruby A (6)
|
MAWWC |
|
DQ |
- |
|
Eruslanova, Elizabeth (6)
|
MASSC |
30.65 |
NS |
- |