| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fleischaker, Mazie R (8)
|
MAPAAC |
46.14 |
42.69 |
-3.45 |
| 2 |
Bobalik, Vivian R (8)
|
MAGRA |
47.67 |
45.60 |
-2.07 |
| 3 |
Berzanski, Audrey D (8)
|
MAJW |
48.39 |
46.04 |
-2.35 |
| 4 |
Mogck, Ella J (8)
|
MAGRA |
|
46.42 |
- |
| 5 |
Ewer, Lily C (8)
|
MAMSA |
51.13 |
46.57 |
-4.56 |
| 6 |
Goldin, Madison R (8)
|
MAJW |
51.44 |
48.34 |
-3.10 |
| 7 |
Kieffer, Margaret K (8)
|
MALAC |
|
48.67 |
- |
| 8 |
Taylor, Reagan E (8)
|
MAACAC |
|
49.04 |
- |
| 9 |
Shaud, Audrey A (8)
|
MASSC |
|
50.54 |
- |
| 10 |
Eruslanova, Elizabeth (8)
|
MASSC |
|
51.50 |
- |
| 11 |
Gopinath, Ovia (8)
|
MAGRA |
|
52.84 |
- |
| 12 |
Henry, Sidney E (7)
|
MAGRA |
1:00.77 |
53.82 |
-6.95 |
| 13 |
Bergmann, Sarah (8)
|
MAACAC |
59.37 |
54.27 |
-5.10 |
| 14 |
Turek, Ellie K (8)
|
MAUNGR |
|
57.40 |
- |
| 15 |
Roberts, Lillian (8)
|
MAACAC |
1:07.71 |
59.20 |
-8.51 |
| 16 |
Oconnor, Claire V (8)
|
MAGRA |
|
59.26 |
- |
| 17 |
Tewksbury, Victoria E (8)
|
MAPAAC |
58.37 |
59.51 |
1.14 |
| 18 |
Sheplock, Jillan (8)
|
MAACAC |
|
1:06.83 |
- |
|
Wilson, Paige S (8)
|
MAGRA |
42.46 |
DQ |
- |
|
Wadke, Raina K (8)
|
MABSC |
|
NS |
- |
|
Benevento, Saoirse L (8)
|
MAWA |
1:04.76 |
NS |
- |