| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stewart, Quinlan M (8)
|
MAWA |
23.94 |
23.92 |
-0.02 |
| 2 |
Berzanski, Audrey D (8)
|
MAJW |
24.06 |
24.04 |
-0.02 |
| 3 |
Mogck, Ella J (8)
|
MAGRA |
|
26.50 |
- |
| 4 |
Tewksbury, Victoria E (8)
|
MAPAAC |
35.08 |
28.36 |
-6.72 |
| 5 |
Ditullio, Kara (8)
|
MAUNAC |
27.30 |
28.71 |
1.41 |
| 6 |
Lightman, Chloe A (8)
|
MABSC |
39.84 |
30.25 |
-9.59 |
| 7 |
McGarry, Lucy E (8)
|
MABSC |
|
30.29 |
- |
| 8 |
Taylor, Reagan E (8)
|
MAACAC |
30.15 |
30.38 |
0.23 |
| 9 |
Hanway, Cathryn T (8)
|
MASSC |
37.88 |
30.43 |
-7.45 |
| 10 |
Barr, Annie (8)
|
MACAA |
|
31.44 |
- |
| 11 |
Galbraith, Maya R (8)
|
MALAC |
|
34.12 |
- |
| 12 |
Gowdy, Madison R (8)
|
MAJW |
34.08 |
35.71 |
1.63 |
| 13 |
Roberts, Lillian (8)
|
MAACAC |
42.03 |
38.02 |
-4.01 |
| 14 |
Bergmann, Sarah (8)
|
MAACAC |
|
41.96 |
- |
| 15 |
Daily, Leah (8)
|
MACAA |
|
52.80 |
- |
|
Ginesin, Celia F (8)
|
MABSC |
34.38 |
DQ |
- |
|
Strachan, Elizabeth D (8)
|
MASSC |
|
DQ |
- |
|
Wadke, Raina K (8)
|
MABSC |
32.39 |
NS |
- |
|
Navarro, Addison (8)
|
MABSC |
36.82 |
NS |
- |
|
Benevento, Saoirse L (8)
|
MAWA |
41.84 |
NS |
- |