| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kibler, Liam D (10)
|
VABASS |
35.32 |
34.77 |
-0.55 |
| 2 |
Kautz-Scanavy, Christian H (10)
|
VASRVA |
36.85 |
36.30 |
-0.55 |
| 3 |
Chapman, Isaac (8)
|
VASRVA |
|
39.63 |
- |
| 4 |
Miller, Anderson E (9)
|
VASRVA |
|
40.31 |
- |
| 4 |
Dilmore, Casey A (10)
|
VASRVA |
41.69 |
40.31 |
-1.38 |
| 6 |
Bell, Lukas B (8)
|
VASRVA |
|
40.39 |
- |
| 7 |
Payne, Atlas R (9)
|
VABASS |
|
41.44 |
- |
| 8 |
DesMarteau, Callan S (9)
|
VASRVA |
47.85 |
42.47 |
-5.38 |
| 9 |
Schiavone, William L (10)
|
VASRVA |
52.93 |
43.73 |
-9.20 |
| 10 |
Aleksashyn, Kyryl (10)
|
VADC |
|
43.87 |
- |
| 11 |
Vonck, Theodore A (9)
|
VASRVA |
53.06 |
44.54 |
-8.52 |
| 12 |
Whitt, Nolan R (8)
|
VASRVA |
|
46.35 |
- |
| 13 |
Wilson, Paxton M (7)
|
VASRVA |
|
48.05 |
- |
| 14 |
Grandis, Cardwell T (9)
|
VADC |
50.81 |
48.64 |
-2.17 |
| 15 |
Schwalm, Lincoln B (8)
|
VASRVA |
|
50.10 |
- |
| 16 |
Logan, Michael D (9)
|
VABASS |
|
50.85 |
- |
| 17 |
Dilmore, Jameson M (8)
|
VASRVA |
55.48 |
50.92 |
-4.56 |
| 18 |
Aleksashyn, Oleksandr (8)
|
VADC |
|
54.63 |
- |
| 19 |
Mexal, Christian T (7)
|
VASRVA |
|
59.50 |
- |
| 20 |
Holland, Landon J (10)
|
VAECAT |
|
1:02.16 |
- |
|
Blount, Jared L (8)
|
VAECAT |
|
NS |
- |
|
Timken, Gage T (10)
|
VABASS |
|
NS |
- |