| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bermudez, Leilani K (11)
|
FGJDST |
42.16 |
40.97 |
-1.19 |
| 2 |
Eskra, Paige K (12)
|
FGNPB |
44.92 |
42.22 |
-2.70 |
| 3 |
Fox, Maria A (12)
|
BSC |
41.32 |
42.64 |
1.32 |
| 4 |
Han, Miranda A (12)
|
FGWOW |
42.18 |
42.83 |
0.65 |
| 5 |
Meyer, Camryn E (12)
|
FGTSA |
43.31 |
43.09 |
-0.22 |
| 6 |
MacKenzie, Alice R (12)
|
FGJDST |
46.41 |
44.81 |
-1.60 |
| 7 |
Macaulay, Anna M (12)
|
FGJDST |
47.88 |
45.13 |
-2.75 |
| 8 |
Baratta, Amanda M (11)
|
FGWOW |
45.97 |
47.02 |
1.05 |
| 9 |
Hidalgo Cadet, Maripaz (11)
|
FGJDST |
50.74 |
48.50 |
-2.24 |
| 10 |
Honzik, Natalie V (11)
|
FGWOW |
50.76 |
49.09 |
-1.67 |
| 11 |
Hartley, Delia J (12)
|
FGNPB |
|
49.54 |
- |
| 12 |
Concho, Camila C (11)
|
FGTSA |
|
49.88 |
- |
| 13 |
Marx, Isabella J (12)
|
FGNPB |
50.45 |
50.52 |
0.07 |
| 14 |
D'Loughy, Fredericka H (12)
|
FGNPB |
|
50.62 |
- |
| 15 |
Gonzalez, Gabriella (11)
|
FGYSPB |
54.74 |
50.67 |
-4.07 |
| 16 |
Wood, Nikki S (11)
|
FGPOMP |
56.70 |
50.73 |
-5.97 |
| 17 |
Velasquez, Isabella (11)
|
FGWOW |
|
51.14 |
- |
| 18 |
Swanson, Katherine V (12)
|
FGNPB |
50.71 |
52.35 |
1.64 |
| 19 |
Rozin, Carla B (12)
|
FGTSA |
1:01.36 |
53.32 |
-8.04 |
| 20 |
Muller, Averie C (12)
|
FGJDST |
1:13.87 |
53.33 |
-20.54 |
| 21 |
Howells, Lillian O (11)
|
FGJDST |
|
53.83 |
- |
| 22 |
Rubio, Sienna S (11)
|
FGJDST |
|
53.93 |
- |
| 23 |
D'Loughy, Wilhelmina M (12)
|
FGNPB |
|
54.63 |
- |
| 24 |
Henry, Teryn (12)
|
FGTSA |
55.98 |
54.71 |
-1.27 |
| 25 |
Thomas, Morgan A (11)
|
FGTSA |
1:16.43 |
1:03.05 |
-13.38 |
| 26 |
Coleman, Abigail G (12)
|
FGJDST |
|
1:04.35 |
- |
| 27 |
Keller, Molly (11)
|
FGJDST |
|
1:05.61 |
- |
|
Wells, Aria K (12)
|
FGNPB |
|
NS |
- |