| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Headley, Mackenzie N (12)
|
CTFVYT |
26.40 |
26.93 |
0.53 |
| 2 |
Henderson, Sarah R (12)
|
NEPVAA |
29.90 |
30.39 |
0.49 |
| 3 |
Kelly, Megan E (12)
|
NEABF |
29.86 |
30.95 |
1.09 |
| 4 |
Brown, Laura M (12)
|
NEHYV |
31.46 |
32.29 |
0.83 |
| 5 |
Villanueva, Sofia R (12)
|
NEPVAA |
36.14 |
35.64 |
-0.50 |
| 6 |
Satkowski, Mia M (12)
|
NEWYST |
33.66 |
35.66 |
2.00 |
| 7 |
Bulatewicz, Mia (12)
|
NEPVAA |
35.88 |
36.32 |
0.44 |
| 8 |
Maltby, Hannah M (12)
|
NEABF |
38.51 |
37.21 |
-1.30 |
| 9 |
Salls, Madelyn R (11)
|
NEWYST |
37.88 |
40.20 |
2.32 |
| 10 |
Dole, Inez S (12)
|
NEHRY |
38.85 |
40.25 |
1.40 |
| 11 |
Luna-Rodriguez, Sophia I (11)
|
NEPVAA |
40.11 |
40.65 |
0.54 |
| 12 |
Hanechak, Elizabeth Z (12)
|
NEWYST |
40.87 |
42.70 |
1.83 |
| 13 |
Kimball, Ellie A (12)
|
NEHRY |
|
43.25 |
- |
| 14 |
Bloomenthal, Clara A (11)
|
NEHRY |
1:00.23 |
58.96 |
-1.27 |