| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sapio, Olivia M (17)
|
NITWST |
51.23 |
53.57 |
2.34 |
| 2 |
Scheu, Makayla C (15)
|
NIHAMB |
56.58 |
56.94 |
0.36 |
| 3 |
Drummond, Riley E (16)
|
NITWST |
53.80 |
57.25 |
3.45 |
| 4 |
Holland, Elle C (16)
|
NITWST |
54.27 |
58.57 |
4.30 |
| 5 |
Orie, Danielle (18)
|
NITWST |
55.78 |
1:01.00 |
5.22 |
| 6 |
Prusaczyk, Victoria (16)
|
NITWST |
1:04.01 |
1:01.87 |
-2.14 |
| 7 |
Polowy, McKayla L (17)
|
NIHHSC |
1:00.58 |
1:02.63 |
2.05 |
| 8 |
Ray, Yana M (15)
|
NIEAST |
1:02.52 |
1:03.65 |
1.13 |
| 9 |
Schumacher, Anna M (15)
|
NIBCSR |
1:05.29 |
1:04.54 |
-0.75 |
| 10 |
Penchaszadeh-Robert, Julia (15)
|
NITTSC |
1:06.45 |
1:06.12 |
-0.33 |
| 11 |
Bogulski, Marin J (15)
|
NITWST |
1:09.25 |
1:08.84 |
-0.41 |
| 12 |
Moss, Heidi J (16)
|
NIPVSC |
1:13.00 |
1:09.35 |
-3.65 |
| 13 |
Baratta, Abby R (15)
|
NIEAST |
1:15.34 |
1:11.65 |
-3.69 |
| 14 |
Ziemba, Sarah J (15)
|
NIHAMB |
1:08.58 |
1:12.24 |
3.66 |
|
Chwalinski, Katlyn A (18)
|
NIHAMB |
1:32.56 |
NS |
- |