| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Scheu, Makayla C (15)
|
NIUN-H |
25.68 |
26.03 |
0.35 |
| 2 |
Pasquarella, Paige E (16)
|
NITWST |
25.96 |
27.11 |
1.15 |
| 3 |
Quinlan, Evelyn (16)
|
NITWST |
26.61 |
28.09 |
1.48 |
| 4 |
Polowy, McKayla L (16)
|
NIHHSC |
28.22 |
29.13 |
0.91 |
| 5 |
Ray, Yana M (15)
|
NIEAST |
30.60 |
30.12 |
-0.48 |
| 6 |
Venturo, Madeline T (15)
|
NITWST |
30.49 |
30.25 |
-0.24 |
| 7 |
Talty, Kathryn R (16)
|
NITWST |
31.23 |
30.40 |
-0.83 |
| 8 |
May, Julia A (15)
|
NIEAST |
32.44 |
30.55 |
-1.89 |
| 9 |
Bogulski, Marin J (15)
|
NITWST |
32.47 |
31.41 |
-1.06 |
| 10 |
Foley, Megan M (15)
|
NIHHSC |
32.25 |
33.31 |
1.06 |
| 11 |
Chwalinski, Katlyn A (17)
|
NIHAMB |
39.66 |
41.12 |
1.46 |