| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Marcyan, Maya G (12)
|
NITTSC |
30.17 |
30.29 |
0.12 |
| 2 |
Bell, Fina M (12)
|
NITTSC |
30.82 |
31.08 |
0.26 |
| 3 |
Griffin, Haley A (11)
|
NITTSC |
35.87 |
33.51 |
-2.36 |
| 3 |
Flickinger, Maria J (11)
|
NIEAST |
47.76 |
42.36 |
-5.40 |
| 4 |
Zawodzinski, Zoe E (11)
|
NIHAMB |
38.69 |
33.77 |
-4.92 |
| 5 |
Kubiak, Kelly A (12)
|
NITWST |
34.70 |
34.67 |
-0.03 |
| 6 |
Roberts, Alaina G (12)
|
NITTSC |
37.44 |
35.81 |
-1.63 |
| 7 |
Latona, Dena M (12)
|
NIHAMB |
37.19 |
36.61 |
-0.58 |
| 8 |
Brinker, Anna (11)
|
NITWST |
36.14 |
36.65 |
0.51 |
| 9 |
Stoelting, Rachel A (11)
|
NITTSC |
38.66 |
37.42 |
-1.24 |
| 10 |
Kottakis, Emma N (11)
|
NITTSC |
38.84 |
37.59 |
-1.25 |
| 11 |
LeBaron, Julia L (11)
|
NIHHSC |
40.47 |
38.03 |
-2.44 |
| 12 |
Rydzewski, Gabriella D (11)
|
NITWST |
44.32 |
39.04 |
-5.28 |
| 13 |
Williams, Cierra M (11)
|
NITTSC |
41.20 |
39.38 |
-1.82 |
| 14 |
Gardiner, Leah J (11)
|
NITTSC |
|
48.14 |
- |
| 15 |
Mancuso, Gabriella J (11)
|
NIHAMB |
55.58 |
52.33 |
-3.25 |
| 16 |
Schmitt, Larrissa R (11)
|
NIHAMB |
1:06.17 |
1:11.65 |
5.48 |
|
Dunn, Mary T (11)
|
NIHHSC |
|
DQ |
- |