| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moriarty, Giovanna R (17)
|
PVMACH |
5:24.38 |
5:30.07 |
5.69 |
| 2 |
Wardinski, Catherine E (16)
|
PVMACH |
5:33.12 |
5:32.24 |
-0.88 |
| 3 |
Herms, Anna M (16)
|
PVMACH |
5:35.81 |
5:34.04 |
-1.77 |
| 4 |
Prince, Holly C (15)
|
PVYORK |
5:57.91 |
5:41.52 |
-16.39 |
| 5 |
Van Dam, Hannah J (17)
|
PVMACH |
5:44.25 |
5:43.24 |
-1.01 |
| 6 |
Chua, Alexus S (16)
|
PVMACH |
5:43.22 |
5:43.54 |
0.32 |
| 7 |
Melnikova, Anastasia Y (15)
|
PVSDS |
6:00.44 |
5:49.57 |
-10.87 |
| 8 |
Kugler, Grace D (16)
|
PVMACH |
5:50.91 |
5:49.72 |
-1.19 |
| 9 |
Harris, Lillian J (15)
|
PVFBST |
5:43.51 |
5:53.17 |
9.66 |
| 10 |
Lenard, Rachel L (15)
|
PVAAC |
5:55.62 |
5:54.04 |
-1.58 |
| 11 |
Fritz, Emily A (15)
|
PVMACH |
6:14.56 |
5:54.47 |
-20.09 |
| 12 |
Malo, Megan N (16)
|
PVPM |
6:02.70 |
5:57.44 |
-5.26 |
| 13 |
Salyers, Camille R (17)
|
PVSDS |
6:09.80 |
5:59.64 |
-10.16 |
| 14 |
Bacon, Meredith J (15)
|
PVCSC |
6:22.48 |
6:09.80 |
-12.68 |
| 15 |
Charles, Elisabeth C (15)
|
PVASA |
6:03.61 |
6:10.19 |
6.58 |
| 16 |
Palomino, Elena S (15)
|
PVPM |
6:39.93 |
6:17.02 |
-22.91 |
| 17 |
Miller, Meredith V (17)
|
PVPM |
6:35.34 |
6:24.74 |
-10.60 |