| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chase, Taylor R (8)
|
NIFKVL |
47.50 |
48.58 |
1.08 |
| 2 |
Smith, Juliana M (8)
|
NISAAC |
58.38 |
49.35 |
-9.03 |
| 3 |
Christensen, Kylee M (8)
|
NIGVST |
55.57 |
49.75 |
-5.82 |
| 4 |
Ground, Jaylee D (8)
|
NISAAC |
53.82 |
54.81 |
0.99 |
| 5 |
Green, Ella A (8)
|
NIGVST |
53.32 |
55.09 |
1.77 |
| 7 |
Owens, Lillian D (8)
|
NIFKVL |
1:02.60 |
57.59 |
-5.01 |
| 8 |
Budinger, Annabelle R (8)
|
NIGVST |
58.60 |
59.62 |
1.02 |
| 9 |
Reed, Alexa R (8)
|
NISAAC |
1:05.26 |
1:03.72 |
-1.54 |
| 10 |
Landow, Kayci L (7)
|
NIFKVL |
1:01.00 |
1:06.35 |
5.35 |
| 11 |
Castellano, Rebecca J (6)
|
NIGVST |
1:11.44 |
1:10.12 |
-1.32 |
| 12 |
Sullivan, Mya M (7)
|
NIFKVL |
1:04.04 |
1:12.18 |
8.14 |
| 13 |
Dougherty, Regina A (7)
|
NIGVST |
1:33.62 |
1:15.52 |
-18.10 |
| 14 |
Haley, Delaney J (6)
|
NISAAC |
1:29.48 |
1:17.97 |
-11.51 |
| 15 |
Bova, Keira E (7)
|
NISAAC |
1:27.14 |
1:18.96 |
-8.18 |
| 16 |
Graham, Gracyn M (6)
|
NIGVST |
1:14.48 |
1:20.67 |
6.19 |
| 17 |
Jacobs, Acelynn K (5)
|
NISAAC |
1:38.88 |
1:24.23 |
-14.65 |
| 18 |
Hunt, Camdyn I (7)
|
NIGVST |
1:40.73 |
1:30.73 |
-10.00 |
|
Owens, Annabelle R (7)
|
NIFKVL |
1:34.76 |
NS |
- |