| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McConnell, Caroline A (6)
|
PVRMSC |
23.10 |
23.87 |
0.77 |
| 2 |
Mejia-Mardeeva, Annabel M (6)
|
PVRMSC |
25.73 |
24.32 |
-1.41 |
| 3 |
Kim, Charlotte Y (6)
|
PVRMSC |
23.77 |
24.70 |
0.93 |
| 4 |
Li, MingZe (6)
|
PVTOLL |
26.33 |
25.10 |
-1.23 |
| 5 |
Nguyen, Sloane M (5)
|
PVMACH |
26.66 |
25.74 |
-0.92 |
| 6 |
Qin, Olivia (6)
|
PVRMSC |
28.17 |
25.89 |
-2.28 |
| 7 |
Chang, Sydney X (6)
|
PVRMSC |
24.40 |
25.97 |
1.57 |
| 8 |
Zettler, Grace A (6)
|
PVASA |
23.01 |
26.16 |
3.15 |
| 9 |
Yun, Clara (6)
|
PVRMSC |
31.46 |
26.89 |
-4.57 |
| 10 |
Netram, Serena R (6)
|
PVRMSC |
28.77 |
28.07 |
-0.70 |
| 11 |
Santos, Alaina (6)
|
PVRMSC |
30.35 |
28.20 |
-2.15 |
| 12 |
Averill, Karina K (6)
|
PVRMSC |
23.64 |
28.35 |
4.71 |
| 13 |
Grijalba, Lucia (6)
|
PVRMSC |
26.81 |
28.46 |
1.65 |
| 14 |
Kwon, Eleanor (6)
|
PVRMSC |
28.12 |
29.42 |
1.30 |
| 15 |
Luongo, Blake A (6)
|
PVCSC |
34.12 |
30.32 |
-3.80 |
| 16 |
He, Athena C (6)
|
PVRMSC |
32.36 |
30.34 |
-2.02 |
| 17 |
Dussault, Eva P (6)
|
PVSDS |
31.31 |
30.78 |
-0.53 |
| 18 |
Thomson, Charlotte F (6)
|
MAJW |
30.17 |
31.88 |
1.71 |
| 19 |
Wilson, Zuri E (6)
|
PVMACH |
|
32.03 |
- |
| 20 |
Wiley-Jimenez, Ariadne M (6)
|
PVFISH |
32.65 |
32.08 |
-0.57 |
| 21 |
Whiteman, Hannah E (6)
|
PVFAST |
32.38 |
36.20 |
3.82 |
| 22 |
Hu, Sophia G (6)
|
PVMSSC |
38.50 |
37.02 |
-1.48 |
| 23 |
Trepkowski, Sabrina N (5)
|
PVRMSC |
35.95 |
37.37 |
1.42 |
| 24 |
McHenry, Ziva Z (6)
|
PVFISH |
40.72 |
41.40 |
0.68 |
| 25 |
Lehman, Ainsley B (6)
|
PVSDS |
31.36 |
42.17 |
10.81 |
|
Bell, Aria G (6)
|
PVRMSC |
33.90 |
DQ |
- |
|
Dmitrova, Stephania Z (5)
|
PVFISH |
|
DQ |
- |
|
Reilly, Clara G (6)
|
PVSDS |
28.61 |
NS |
- |