| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lopez, Chelsea E (6)
|
PVTIBU |
42.93 |
40.07 |
-2.86 |
| 2 |
Pelaez, Riley K (6)
|
PVFISH |
43.09 |
40.50 |
-2.59 |
| 3 |
Scott, Elizabeth G (6)
|
PVFISH |
47.77 |
45.17 |
-2.60 |
| 4 |
Chen, Livia (6)
|
PVRMSC |
47.61 |
45.37 |
-2.24 |
| 5 |
Flaim, Isabella J (6)
|
PVRMSC |
47.25 |
46.26 |
-0.99 |
| 6 |
Ngo, Nhi K (6)
|
PVSA |
59.20 |
46.40 |
-12.80 |
| 7 |
Wellen, Charlotte G (6)
|
PVRMSC |
48.36 |
46.50 |
-1.86 |
| 8 |
Wang, Hailey (6)
|
PVRMSC |
44.37 |
47.25 |
2.88 |
| 9 |
Diener, Tatiana Q (6)
|
PVRMSC |
52.79 |
50.12 |
-2.67 |
| 10 |
Taylor, Jovie M (6)
|
PVRMSC |
52.91 |
53.08 |
0.17 |
| 11 |
Song, Evelyn M (6)
|
PVRMSC |
59.02 |
53.71 |
-5.31 |
| 12 |
Mahaffy, Caroline A (6)
|
PVFISH |
|
1:20.78 |
- |
| 13 |
Albino-Griffith, Gianna M (5)
|
PVFISH |
1:33.78 |
1:25.22 |
-8.56 |
| 14 |
Warner, Alyse (5)
|
PVRMSC |
|
1:34.82 |
- |
| 15 |
Moore, Avery J (5)
|
PVASTS |
2:28.38 |
1:42.19 |
-46.19 |
|
Cotner Phillips, Lili'u Aloh (6)
|
PVFISH |
|
DQ |
- |
|
Forsythe, Alexandra K (6)
|
PVFISH |
54.11 |
DQ |
- |
|
Burnayeva, Keira (6)
|
PVRMSC |
|
NS |
- |
|
Wert, Genevieve E (6)
|
PVRMSC |
54.31 |
NS |
- |
|
Beinecke, Samantha K (6)
|
PVRMSC |
1:01.54 |
NS |
- |