| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chen, Livia (6)
|
PVRMSC |
25.92 |
25.86 |
-0.06 |
| 2 |
Lopez, Chelsea E (6)
|
PVTIBU |
26.46 |
27.61 |
1.15 |
| 3 |
Scott, Elizabeth G (6)
|
PVFISH |
26.59 |
28.13 |
1.54 |
| 4 |
Pelaez, Riley K (6)
|
PVFISH |
28.93 |
30.98 |
2.05 |
| 5 |
Wang, Alice R (6)
|
PVRMSC |
31.28 |
32.21 |
0.93 |
| 6 |
Diener, Tatiana Q (6)
|
PVRMSC |
29.64 |
32.38 |
2.74 |
| 7 |
Taylor, Jovie M (6)
|
PVRMSC |
34.44 |
34.72 |
0.28 |
| 8 |
Wang, Hailey (6)
|
PVRMSC |
|
35.37 |
- |
| 9 |
Masaya Golovko, Alexandra J (6)
|
PVRMSC |
33.45 |
36.16 |
2.71 |
| 10 |
Wellen, Charlotte G (6)
|
PVRMSC |
32.24 |
37.24 |
5.00 |
| 11 |
Song, Evelyn M (6)
|
PVRMSC |
33.69 |
37.44 |
3.75 |
| 12 |
DeShayes, Eliza L (6)
|
PVRMSC |
36.83 |
40.07 |
3.24 |
| 13 |
Hongola, Lorelai (6)
|
PVTIBU |
52.25 |
43.93 |
-8.32 |
|
Mejia-Mardeeva, Isabel R (6)
|
PVRMSC |
38.12 |
DQ |
- |
|
Ngo, Nhi K (6)
|
PVSA |
|
DQ |
- |
|
Parlock, Abigail R (6)
|
PVJFD |
28.03 |
DQ |
- |
|
Moore, Avery J (5)
|
PVASTS |
|
NS |
- |
|
Burnayeva, Keira (6)
|
PVRMSC |
|
NS |
- |
|
Bolomsky-Lutz, Ilvy V (6)
|
PVRMSC |
26.39 |
NS |
- |
|
Beinecke, Samantha K (6)
|
PVRMSC |
45.83 |
NS |
- |
|
Wert, Genevieve E (6)
|
PVRMSC |
35.33 |
NS |
- |