| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cooper, Caitlin M (20)
|
VACAV |
56.61 |
56.36 |
-0.25 |
| 2 |
Gruber, Margaret A (20)
|
VAHOKI |
57.02 |
56.92 |
-0.10 |
| 3 |
Henig, Isabelle M (15)
|
PCPASA |
56.60 |
56.95 |
0.35 |
| 4 |
Fackenthal, Amalie K (16)
|
SNDART |
57.27 |
57.04 |
-0.23 |
| 5 |
Hayden, Rachel E (20)
|
MVUMIZ |
56.90 |
57.93 |
1.03 |
| 6 |
Anderson, Gabrielle N (16)
|
PCPASA |
58.18 |
58.19 |
0.01 |
| 7 |
Reese, Emily E (16)
|
GUMAC |
58.10 |
58.71 |
0.61 |
| 8 |
Fullenkamp, Nora G (17)
|
OHDR |
58.65 |
58.96 |
0.31 |
| 9 |
Cuomo, Alexa M (15)
|
PVMAKO |
59.12 |
59.14 |
0.02 |
| 10 |
Debeer, Tonner E (16)
|
KYTS |
58.38 |
59.17 |
0.79 |
| 11 |
Maiocco, Claire O (17)
|
FLTPA |
58.15 |
59.36 |
1.21 |
| 12 |
Donohoe, Madelyn E (14)
|
PVFISH |
59.00 |
59.39 |
0.39 |
| 13 |
Grover, Claire R (16)
|
AZSAC |
57.99 |
59.59 |
1.60 |
| 14 |
Morris, Halle M (17)
|
PCNBA |
1:01.50 |
1:00.32 |
-1.18 |
| 15 |
McCauley, Ashley E (15)
|
NCMOR |
1:01.99 |
1:01.42 |
-0.57 |