| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McQuet, Murphy J (17)
|
ILNTSC |
|
50.58 |
- |
| 2 |
Fotsch, Connor W (18)
|
ILNTSC |
|
50.84 |
- |
| 3 |
Sesvold, Tyler E (15)
|
ILUN |
|
51.03 |
- |
| 4 |
Kapphahn, Alexander J (17)
|
SNUN |
|
51.81 |
- |
| 5 |
Bean, Clifford B (18)
|
MDNAAC |
|
52.06 |
- |
| 6 |
Digiacomo, Christopher L (17)
|
NEAAC |
|
52.14 |
- |
| 7 |
Barrett, Craig D (17)
|
NEAAC |
|
52.87 |
- |
| 8 |
Kostboth, Kody R (18)
|
MVWSC |
|
53.05 |
- |
| 8 |
Bosse, Nathan T (17)
|
ILPAC |
|
53.05 |
- |
| 10 |
Keenan, Drew (16)
|
ILNTSC |
|
53.09 |
- |
| 10 |
Lee, Max C (18)
|
GADYNA |
|
53.09 |
- |
| 10 |
Elkington, Matthew L (16)
|
CCCLOV |
|
53.09 |
- |
| 13 |
Kranenburg, Stefan P (18)
|
PCUN-0 |
|
53.28 |
- |
| 14 |
Nabors, Seth L (16)
|
CCCLOV |
|
53.81 |
- |
| 15 |
Noel, Jake W (16)
|
ILMMSC |
|
54.01 |
- |
| 16 |
Welty, Tyler L (16)
|
OHNAAC |
|
54.44 |
- |
| 17 |
Andrews, Theo J (15)
|
PCNBA |
|
54.98 |
- |
| 18 |
Amitay, Ethan (16)
|
PVRMSC |
|
55.36 |
- |
|
Rich, Justin R (16)
|
VAPSDN |
|
NS |
- |