| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Holmes, Camden M (16)
|
MEDEFY |
47.35 |
47.67 |
0.32 |
| 2 |
Prouty, Carson J (15)
|
MECCSC |
48.99 |
47.89 |
-1.10 |
| 3 |
Yost, Matthew E (16)
|
MELRSC |
49.27 |
49.09 |
-0.18 |
| 4 |
Sullivan, Liam J (16)
|
MEMDIY |
48.78 |
49.31 |
0.53 |
| 5 |
Schnetzer, William R (17)
|
MEWCY |
48.84 |
49.42 |
0.58 |
| 6 |
Johnson, Brandon D (16)
|
MELRSC |
52.18 |
50.13 |
-2.05 |
| 7 |
O'Farrell, William C (17)
|
MEPBAY |
50.45 |
50.59 |
0.14 |
| 8 |
Overlock, Ethan Z (15)
|
MEKVY |
51.10 |
51.80 |
0.70 |
| 9 |
Witham, Jacob R (16)
|
MEMMD |
51.04 |
52.64 |
1.60 |
| 10 |
Whitman, Brendan M (16)
|
METCST |
52.84 |
53.10 |
0.26 |
| 11 |
Gurney, Alexander E (16)
|
MELRSC |
54.94 |
53.26 |
-1.68 |
| 12 |
Hedstrom, Connor D (16)
|
MEPBAY |
51.96 |
54.32 |
2.36 |
| 13 |
Hyde, Robert L (15)
|
MELRSC |
53.84 |
54.54 |
0.70 |
| 14 |
Girard, Lucas R (16)
|
MENYCY |
55.12 |
55.60 |
0.48 |
| 15 |
Orchow, Maxwell J (15)
|
MEPYPB |
56.40 |
55.66 |
-0.74 |
| 16 |
Kristan, Rowan (15)
|
MEBBYD |
58.29 |
56.34 |
-1.95 |
| 17 |
Bishop, Patrick H (15)
|
MEPYPB |
58.41 |
58.48 |
0.07 |
| 18 |
Frumiento, Iain (17)
|
METCST |
1:00.16 |
59.08 |
-1.08 |
| 19 |
Ragg-Schmidt, Matt (18)
|
MEKVY |
1:04.48 |
1:00.10 |
-4.38 |
| 20 |
McCoy, Jordan (18)
|
MECCSC |
1:00.93 |
1:01.11 |
0.18 |
| 21 |
Corrigan, Owen M (17)
|
MEMMD |
1:01.88 |
1:02.17 |
0.29 |
| 22 |
Ciano, Christopher (16)
|
MEDEFY |
1:05.29 |
1:03.45 |
-1.84 |
| 23 |
Facq, Alexander D (16)
|
MEPBAY |
1:09.08 |
1:04.98 |
-4.10 |
| 24 |
Grabowski, Elias (13)
|
MENYCY |
1:10.87 |
1:06.36 |
-4.51 |
| 25 |
Bradenday, Oakley B (14)
|
MEPYPB |
1:13.10 |
1:09.91 |
-3.19 |
| 26 |
Rancourt, Nicholas V (17)
|
MEMMD |
1:11.01 |
1:10.84 |
-0.17 |
| 27 |
Ladakakos, Logan J (15)
|
MENYCY |
1:11.99 |
1:13.70 |
1.71 |
|
Blackstone, Ian D (17)
|
MEPRY |
1:00.50 |
NS |
- |