| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tarolli, Vincent M (15)
|
NICSCI |
51.48 |
52.04 |
0.56 |
| 2 |
Shelmidine, Nolan E (16)
|
NIMETS |
57.47 |
56.09 |
-1.38 |
| 3 |
Burke, Connor D (13)
|
NISYCH |
58.03 |
57.82 |
-0.21 |
| 4 |
Diel, Robert M (15)
|
NISYCH |
1:00.12 |
57.91 |
-2.21 |
| 5 |
Van Kersbergen, Gavin M (11)
|
NISKAN |
57.02 |
58.39 |
1.37 |
| 6 |
Williams, Jonah F (12)
|
NISKAN |
1:01.37 |
58.86 |
-2.51 |
| 7 |
Miller, Treyse B (12)
|
NIMETS |
1:04.91 |
1:03.83 |
-1.08 |
| 8 |
Denkenberger, Quinten M (13)
|
NIOLSC |
1:02.87 |
1:04.00 |
1.13 |
| 9 |
Monroe, Lucas J (13)
|
NIPMSC |
1:05.32 |
1:06.70 |
1.38 |
| 10 |
Walz, Nathan A (13)
|
NISYCH |
1:07.39 |
1:07.02 |
-0.37 |
| 11 |
Hatch, Collin M (13)
|
NIMETS |
1:18.89 |
1:09.00 |
-9.89 |
| 12 |
Finnerty, Sean P (13)
|
NICSCI |
1:13.60 |
1:09.77 |
-3.83 |
| 13 |
Cole, Matthew J (14)
|
NIOLSC |
1:10.78 |
1:09.91 |
-0.87 |
| 14 |
Newsome, George W (11)
|
NIPMSC |
1:26.76 |
1:10.91 |
-15.85 |
| 15 |
Loomis, Jesse J (13)
|
NIMETS |
1:26.08 |
1:25.37 |
-0.71 |
| 16 |
Klock, Conner G (11)
|
NIJETS |
1:28.94 |
1:29.20 |
0.26 |
| 17 |
Hatch, Lincoln E (11)
|
NIMETS |
|
2:00.52 |
- |
|
Ignacio, Emmanuel T (15)
|
NIOLSC |
1:00.54 |
NS |
- |