| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Clark, Brynn (11)
|
RR |
29.51 |
28.96 |
-0.55 |
| 2 |
Hoger, Paige (11)
|
IADSMY |
33.74 |
35.02 |
1.28 |
| 3 |
Cox, Lexi (12)
|
RR |
35.27 |
36.00 |
0.73 |
| 4 |
Richardson, Caroline (11)
|
IAWASH |
|
36.15 |
- |
| 5 |
Hoger, Lilly (11)
|
IADSMY |
41.89 |
36.79 |
-5.10 |
| 6 |
Dolan, Ryann (11)
|
IAWASH |
36.74 |
36.97 |
0.23 |
| 7 |
Wulf, Allie (11)
|
IAWASH |
39.59 |
40.59 |
1.00 |
| 8 |
Murphy, Maya (12)
|
IAWASH |
38.09 |
40.95 |
2.86 |
| 9 |
Joiner, Jaxton (11)
|
RR |
44.47 |
42.94 |
-1.53 |
| 10 |
Whitney, Brenna (11)
|
RR |
44.76 |
44.18 |
-0.58 |
| 11 |
Carlton, Mallory L (11)
|
IADSMY |
46.77 |
48.43 |
1.66 |
| 12 |
Baeza, Paloma (11)
|
IAOTT |
50.81 |
54.18 |
3.37 |