| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ramos, Daniela I (8)
|
NIUNAC |
29.47 |
28.52 |
-0.95 |
| 2 |
Peet, Christina M (8)
|
NICSST |
30.40 |
29.35 |
-1.05 |
| 3 |
Welliver, Charlotte P (8)
|
NICSST |
|
29.58 |
- |
| 4 |
Liu, Erika (8)
|
NIHAWK |
|
30.68 |
- |
| 5 |
Denlinger, Elizabeth J (8)
|
NIWAAC |
35.14 |
31.52 |
-3.62 |
| 6 |
Wojciechowski, Ella G (8)
|
NIHHTA |
|
31.90 |
- |
| 7 |
Potter, Sadie G (8)
|
NICSST |
|
32.41 |
- |
| 8 |
Hagan, Bridget D (8)
|
NICSST |
31.55 |
32.45 |
0.90 |
| 9 |
Cavalier, Madelyn B (8)
|
NICSST |
37.49 |
32.50 |
-4.99 |
| 10 |
Agosto, Aleida L (8)
|
NICSST |
|
36.85 |
- |
| 11 |
Shutter, Sophie (7)
|
NIHHTA |
|
39.53 |
- |
| 12 |
Jefferis, Frida R (7)
|
NIISWM |
43.60 |
40.07 |
-3.53 |
| 13 |
Klemann, Aubrey s (7)
|
NIGGST |
|
41.19 |
- |
|
Rice, Juliana M (8)
|
NICVSC |
|
DQ |
- |
|
Bucci, Giavanna (7)
|
NICVSC |
44.27 |
DQ |
- |
|
Fryburger, Grace L (8)
|
NIGGST |
|
DQ |
- |
|
Hempel, Emily F (8)
|
NIGGST |
|
DQ |
- |
|
Neubert, Erin A (6)
|
ADSIDN |
|
DQ |
- |
|
Knowlton, Ava L (8)
|
NIHHTA |
|
DQ |
- |
|
Tyler, Natalie L (7)
|
NICAGO |
50.50 |
DQ |
- |
|
Leahey, Emme A (7)
|
NIUNAC |
|
DQ |
- |
|
Collier, Reese A (7)
|
NICSST |
38.82 |
DQ |
- |