| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cucchiaro, Alexandra M (16)
|
MDGBSA |
3:08.40 |
3:04.71 |
-3.69 |
| 2 |
Mead, Mallory J (17)
|
MDLBA |
3:15.63 |
3:05.41 |
-10.22 |
| 3 |
Woods, Mary D (17)
|
MDGBSA |
3:13.35 |
3:14.16 |
0.81 |
| 4 |
Hendrickson, Margaret R (15)
|
MDEST |
3:09.93 |
3:16.87 |
6.94 |
| 5 |
Cattaneo, Isabella M (16)
|
MDLBA |
3:08.17 |
3:17.55 |
9.38 |
| 6 |
Briggs, Emily R (16)
|
MDEST |
4:04.99 |
3:22.56 |
-42.43 |
| 7 |
Smith, Aria C (17)
|
MDGBSA |
3:12.69 |
3:23.12 |
10.43 |
| 8 |
Holiday, Jordin D (15)
|
MDBCSC |
3:26.56 |
3:29.47 |
2.91 |
| 9 |
Nelson, Sarah L (16)
|
MDBAAC |
3:31.83 |
3:39.85 |
8.02 |
| 10 |
Donegan, Angela D (16)
|
MDSPRC |
3:41.34 |
3:42.75 |
1.41 |
| 11 |
Harrington, Quinn L (16)
|
MDMAS |
|
3:50.47 |
- |
| 12 |
Mazur, Karolina T (15)
|
MDEST |
3:34.46 |
3:51.95 |
17.49 |
| 13 |
Taylor, Anna E (16)
|
MDBAAC |
3:45.23 |
3:53.98 |
8.75 |