| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lionel, Caidyn (12)
|
MDBAY |
34.67 |
35.06 |
0.39 |
| 2 |
Asekun, Similoluwa A (12)
|
MDRAC |
36.96 |
37.56 |
0.60 |
| 3 |
Brooks, Khloe A (12)
|
MDBAY |
39.08 |
39.67 |
0.59 |
| 4 |
Azur, Eryn C (12)
|
MDEST |
41.27 |
39.98 |
-1.29 |
| 5 |
Ross, Olivia L (12)
|
MDLBA |
39.88 |
40.02 |
0.14 |
| 6 |
Willis, Haley J (12)
|
MDBAY |
41.20 |
41.42 |
0.22 |
| 7 |
Metzger, Hannah L (12)
|
MDSPY |
41.72 |
41.47 |
-0.25 |
| 8 |
Lamb, Tice E (12)
|
MDLBA |
40.62 |
41.48 |
0.86 |
| 9 |
Alig, Whitney D (12)
|
MAPDR |
45.82 |
42.16 |
-3.66 |
| 10 |
Ryan, Molly K (11)
|
MDEST |
44.27 |
42.31 |
-1.96 |
| 11 |
Jung, Hannah C (12)
|
MDEST |
41.68 |
43.35 |
1.67 |
| 12 |
Scott, Marie-Rose C (11)
|
MAPDR |
45.19 |
43.49 |
-1.70 |
| 13 |
Adams, Reese A (11)
|
MDRAC |
49.57 |
46.33 |
-3.24 |
| 14 |
Patel, Asha C (11)
|
MDRAC |
|
46.35 |
- |
| 15 |
Kalu, Faith (12)
|
MDBAY |
51.08 |
46.46 |
-4.62 |
| 16 |
Vanhorn, Nora M (12)
|
MDBAY |
47.15 |
46.57 |
-0.58 |
| 17 |
Agwamba, Janine C (11)
|
MAPDR |
|
46.65 |
- |
| 18 |
Mollel, Cyra (11)
|
MDBAY |
|
47.19 |
- |
| 19 |
Ghandi, Rana (11)
|
MDEST |
47.77 |
47.35 |
-0.42 |
| 20 |
Hanson, Grace A (11)
|
MDGTAC |
47.77 |
48.00 |
0.23 |
| 21 |
Erb, Alexandra K (11)
|
MAPDR |
|
48.98 |
- |
| 22 |
Bankard, Lily F (12)
|
MDGTAC |
49.42 |
49.45 |
0.03 |
| 23 |
Raeder, Ava (12)
|
MDBAY |
49.96 |
49.48 |
-0.48 |
| 24 |
VanWright, Parker R (11)
|
MAPDR |
52.88 |
51.02 |
-1.86 |
| 25 |
Lewis, Reagan Q (11)
|
MDBAY |
48.68 |
51.09 |
2.41 |
| 26 |
Bundy, Addison R (12)
|
MDJCC |
54.71 |
55.42 |
0.71 |
| 27 |
Dranov, Viviana N (11)
|
MDJCC |
|
56.46 |
- |
| 28 |
Plunkett, Abigail L (12)
|
MDRAC |
|
57.01 |
- |
| 29 |
Wajbel, Emily C (11)
|
MDLBA |
1:12.10 |
1:16.27 |
4.17 |
|
Walters, Madeleine T (12)
|
MAPDR |
32.76 |
NS |
- |