| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McMahon, Kylie M (16)
|
MDNAAC |
5:35.90 |
5:31.23 |
-4.67 |
| 2 |
Barrett, Christina S (16)
|
MDGBSA |
5:40.74 |
5:36.57 |
-4.17 |
| 3 |
Ducey, Cameron D (17)
|
MDEST |
5:35.30 |
5:38.80 |
3.50 |
| 4 |
Luljak, Julia C (15)
|
MDMSC |
5:53.86 |
5:47.75 |
-6.11 |
| 5 |
Gallaudet, Laurel M (15)
|
MDNAAC |
5:55.41 |
5:48.60 |
-6.81 |
| 6 |
Rapuano, Lily M (13)
|
MDEST |
5:54.16 |
5:49.28 |
-4.88 |
| 7 |
Beuchel, Katherine M (14)
|
MDRAC |
5:52.13 |
5:49.45 |
-2.68 |
| 8 |
McCoy, Emily L (15)
|
MDNAAC |
5:39.99 |
5:51.14 |
11.15 |
| 9 |
Gunawardane, Shivanthi (17)
|
MDEST |
5:49.52 |
5:53.69 |
4.17 |
| 10 |
Sand, Elizabeth F (14)
|
MDEST |
5:57.65 |
5:59.76 |
2.11 |
| 11 |
Mar, Sarah E (16)
|
MDRAC |
5:49.71 |
6:00.46 |
10.75 |
| 12 |
Mester, Delaney F (14)
|
MDEST |
5:59.81 |
6:01.04 |
1.23 |
| 13 |
Shaffrey, Hannah M (13)
|
MDEST |
6:00.44 |
6:03.99 |
3.55 |
| 14 |
Lotze, Annika M (13)
|
MDUN |
5:31.59 |
6:10.06 |
38.47 |
| 15 |
Patz, Sophia N (13)
|
MDSPRC |
6:03.96 |
6:16.21 |
12.25 |
| 16 |
O'Connell, Johnna C (15)
|
MDCBAC |
5:56.75 |
6:17.88 |
21.13 |
| 17 |
Bowes, Sabrina C (13)
|
MDEST |
6:27.94 |
6:21.15 |
-6.79 |
| 18 |
Cattaneo, Sophia C (14)
|
MDSPRC |
6:03.80 |
6:29.25 |
25.45 |
| 19 |
Denault, Mikayla M (14)
|
MDHFY |
6:47.04 |
6:52.06 |
5.02 |